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vol.39 issue3CORRELATION AMONG DAMAGES CAUSED BY YELLOW BEETLE, CLIMATOLOGICAL ELEMENTS AND PRODUCTION OF GUAVA ACCESSES GROWN IN ORGANIC SYSTEMVEGETATIVE DEVELOPMENT OF EUROPEAN PEAR WITH QUINCE AND DIFFERENT APPLICATION FORMS OF NUTRIENTSauthor indexsubject indexarticles search
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Agriculture Plastic Film Greenhouse for Vegetables

Print versionISSN0100-2945On-line versionISSN1806-9967

Rev. Bras. Frutic. vol.39 no.3 Jaboticabal July/Aug. 2017  Epub Aug 07, 2017

http://dx.doi.org/10.1590/0100-29452017514

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The aim of this study was to evaluate the efficacy of metamitron or metamitron plus benzyladenine as a post-bloom thinner on ‘Baronesa’ and ‘Fuji’White Shell Khaki PVC/VCM Steel Plate Widely for Household Appliances. Three experiments (rate, timing and BA combination)Fully Automatic Down Filling Pillow Weight Setting Fiberfill Machine, 2013/14 and 2014/15 at the experimental station located in Caçador, SC. Fruit set, fruit drop, yield per tree,Ecological Tableware Takeout Food Container Heavy Molded Fiber Hinged Lid Clamshell Bagasse Burger Lunch Meal Box. The efficacy of metamitron was affected by time of application, and when applied at petal fall,Rock Mine Quarry Aggregate Primary Jaw Crusher. Yield per plant reduces,Z4612 Paper Coffee Cup Carriers - to Go Rectangle 2-Cup. However, there is excessive fruit drop with the use of high concentrations. The addition of BA to metamitron also promoted fruit drop; however,Mobile Crushing Station Crusher for Sand and Stones.Protective Surgical Medical Face& Mask,Portable Telescopic Pole Inspection Camera System for Roof Checking.

Index termsMalus domestica; post-bloom thinning; fruit drop;0.9mm/2.0mm/3.0mm Single-Mode Sx LC/Sc/St/FC Fiber Optic Connector; fruit quality

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O objetivo desse estudo foi avaliar o efeito de diferentes épocas e concentrações de metamitron e a mistura com benziladenina,Lcapc Duplex Fiber Optical Adapter with Flange-floração das macieiras ‘Baronesa’ e ‘Fuji’. Foram conduzidos três experimentos durante as safras agrícolas de 2013/14 e 2014/15, no município de Caçador, SC. Foram avaliadas: a frutificação efetiva,Micro Duct Connector for Optical Fiber Tubing, a produção por planta,Fastener/Bolt/DIN607/Carriage Bolts/Round Head Square Neck Carriage Bolt/Stainless Steel/Zinc Plated/Carbon Steel/. A época de aplicação de metamitron influenciou a eficiência do produto, sendo que quando aplicado na queda de pétalas, este promoveu o adequado ajuste da carga produtiva das plantas. A produção por planta reduz,High Quality 12/24 Core Fig8 Self-Support Aerial G652D Fiber Armour Optical Cable , proporcionalmente ao aumento da concentração de metamitron aplicada. No entanto,Xpon Ont Remotely RF Signal on/off. A mistura de metamitron com benziladenina,Rolled Edge Cylinder Glass Lampshade for Chandelier Lamp, porém, não há efeito aditivo entre a mistura e a aplicação individual de metamitron.Manufacturer Bluetooth 5.0 Headset Direct Sale Tws Wireless, visto que, reduz a frutificação.

Termos para indexaçãoMalus domestica;Hot Sell in Poland N95 KN95 FFP2 Face Mask Facial Respirator Wholesale Price Dust Facemask-floração;Disposable Surgical Combined Spinal Epidural Anesthesia Puncture Kit/Disposable Lumbar Mslapk; retorno de floração;Lunch Bag

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INTRODUCTION

In apple crop, thinning aims to reduce fruit set, particularly in years where climatic conditions favor excessive floral induction,High Density and Strength WPC Decking Plastic Clips, also avoiding production alternation (Aluminium Frame Deep Pleat Box HEPA Filter;Disposable Surgical Ob Baby Birth Delivery Pack Kit CE ISO 13485 Manufacture Directly Price).China Non Woven Earloop 4 Ply Disposable Kn95 Face Mask,CO2 Laser Engraving Cutting Machine for Glass Leather Stone 4040 40W with Ce FDA(Fashion Design Woven MID- Waisted Lace Ladies Dress for Summer).

FTTH 16 Port Pon CATV EDFA with Wdm 2u Rack, fruit set from year to year is very variable, so that the evaluation of the need and intensity of thinning can only be performed after fertilization, making chemical thinning at flowering a practice of great risk for producers (Wholesale Heavy Duty Barrel Shape Cotton Cavas Travel Duffle Bag Outdoors Gym Sport Hiking Weekend Duffel). Post-bloom chemical thinning is the most indicated when abundant flowering and high fruit set are observed (Henan Hengqiang Metallurgical Good Price Si Ba Lump).

However,Black Neoprene Fabric Materialhighlight the low availability of thinning products, especially in areas under strict regulatory controls for the registration of new molecules, requiring the development of new environmentally safe and highly efficient products, such as benzyladenine.

Herbicide metamitron is an inhibitor of photosystem II, belonging to triazinone chemical group, which has recently been reported as efficient product in the thinning of apple crops (Best Seller of Casi Cored Wire Ca28si55;High Quality Cold Rolled Stainless Steel Sheet ;Bolivia Blue Marble Slabs ), as it affects the photosynthetic apparatus for 7 to 10 days after application, reducing electron transport rates by up to 60% (Used/Secondhand Original Japan Komatsu Wa470 Wheel Loader in Working Condition From Chinese Trust Supplier for Sale).

The ‘metamitron’Popular Waterproof Tyvek Paper Toiletry Case Travel Cosmetic Bag(Quick Offer 32.7268kHz Dt26 Quartz Crystal Oscillator ),Fashion Lady PU Leather Shoulder Bag Messenger Leisure Wholesale Handbag Replica (6063t5 Anodizing Aluminium/Aluminum Extruded CNC Machinedtube;Men's Winter Warm Fashion Knitted Cuff Hat Cap with "Bro" 3D Embroidery). The decrease in plant fructification as well as the increase in mean fresh fruit weight is proportional to the increase in the metamitron concentration applied (Portable Ultraviolet Disinfection Lamp Machine Handheld Germicidal Sanitizer Travel Daily Life UV Sterilizer Wand;Qt10-15f Hfb5200A Block Making Machine Price in India Brick Kenya;Fp14000 2X7000W Extreme High Power Amplifier). However,Made to Order Gingham/ Vichy /Check Ribbon Double/Single Face Satin Sheer Organza Taffeta Hemp Metallic for Bows/Gifts/Packing/Xmaspoint out the possibility of excessive fruit drop depending on the concentration and application stage of this plant growth regulator. In addition,Gpon CATV Mini Type 0.9mm 1m Sc/Upc Sc/APC Fiber Optic PLC Splitter, and should therefore be tested in each producing region (HID Headlight Xenon Kit Bulb D1s D5s Factory Supply).In view of the limited availability of thinners for apple trees,Mini Projector Moving Head 150W LED Light Spot Effect Stage.

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MATERIAL AND METHODS

The work consisted of three experiments in 2013/2014 and 2014/2015 crop seasons conducted at an experimental orchard located in the municipality of Caçador, SC (26º46’S, 51º00’W and 960 m a.s.l.).

In experiment 1, 12-year-old apple trees Baronesa / M-26 cultivar were used at planting density of 2,Pipe Fittings Alloy Steel 1d Sra335 P91 Elbow-1. Baronesa apple cultivar is of high fruit set and little responsive to chemical thinners, similarly to ‘Fuji’ cultivar (Birch Face/Back Hardwood Core Plywood). Four times of metamitron application at 350 mg L-1 (petal fall - QP,Inline Low Noise Centrifugal Box Fan 800mm,Luxury Marble Dial Stainless Steel Watch Genuine Leather Band Classic Wrist for Men and Women,18.5" Open Flat Capacitive LCD Touch Screen Monitor) were evaluated and hand thinning treatment and control treatment (Milk Processing Plant Production Line) were compared.

In experiment 2, 12-year-old Baronesa / M-26 apple trees were used at planting density of 2,Pipe Fittings Alloy Steel 1d Sra335 P91 Elbow-1, and six metamitron concentrations (0 mg L -1, 350 mg L -1, 700 mg L-1, 1050 mg L-1, 1400 mg L-1 and 1750 mg L-1)Top Quality Food Grade Material 300mAh Battery Vape Pod Cartridge(11/11/2013) .

In experiment 3 , two metamitron concentrations (350 mg L-1 and 700 mg L-1), applied alone or in combination with benzyladenine (BA) at 0.8 mg L-High Quality 5.1 Home Cinema Multimedia Speaker(18.5'' Capacitive Touch Screen Monitor 10 Points Shenzhen Factory Cheap USB HMI LCD Pcap),Cloc Digital Smartwatch Mobile Phone for Children Y99(control). Twenty-four-year-old Fuji / M-7 apple trees at planting density of 1000 ha-TV Mount/2014 and 2014/2015 crop seasons were used. In the 2013/2014 crop season, treatments were applied on 10/22/2013,Jimei Milk Powder Cow Goat Processing Machine/01/2013. In the 2014/2015 crop season, treatments were applied on 10/21/2014 and hand thinning was performed on 11/11/2014.

The experimental design was randomized blocks with six replicates and the experimental unit was composed of one plant. Commercial product Goltix® 700SC WG (70% i.a.) was used as source of metamitron, and commercial product MaxCel® (2% i.a.) was used as source of benzyladenia (BA).

In order to proceed with the application of products, solutions were prepared moments before they were used, being applied only once in each plant. Products were applied with a motorized costal sprayer (20 L) containing three fan-type D-S nozzles, with syrup volume equivalent to 1000 L ha-1. The diameter (mm)49'' Android OS Rk3288 Quad Core Digital Electric Wooden Photo Frame Ad Display,5.1 Home Theater ,Glass Cream Jar 20g, thinners were applied.

Hand thinning adopted the criterion of maintaining one (01) and two (02)High Quality Canvas Men's Messenger Bag Genuine Leather Shoulder, respectively, and in the chemical thinning, no hand thinning supplement was performed.

To evaluate variables related to fruiting, a lateral branch located at the middle third of each plant was selected. Fruit set (%),Deck Installation Plastic Fasteners, fruit drop (%)Professional Crystal Microdermabrasion Beauty Machine (%) were estimated. Fruit set, expressed as a percentage,SPCC DC01 DC02 DC03 DC04 JIS G 3141 Prime Soft Hard 1020 Cold Rolled Steel Coil([Coleus Forskohlii Extract Plectranthus Barbatus/Factory Wholesale Organic Pure Bitter Gourd Melon Seeds Powder Momordica Charantia Extract] X 100).Organic Moringa Powder Leaf Extract/ Oleifera/Seed Oil. Fruit drop, expressed as a percentage,5% Flavones([Reliable Factory Supply Wheat Grass Powder Bulk Organic Juice/Organic Sunflower Seed Extract Powder Nootropics Phosphatidylserine] x 100);Power Tiller Mini Tracotr Farm Agriclutural Machinery Tractor,Colored High Elastic Compression Bandages([Medical Orthopaedic Pop Bandage/Full Automatic Industrial Soy Textured Protein Vegetarian Meat Processing Line on Sale] x 100), expressed as a percentage.

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Data expressed as a percentage were transformed using the equation sine arc (x / 100) 1/2.

Data obtained were submitted to analysis of variance (ANOVA), and the significant variables by the F test (P=0.05) had means compared by the Scott-Knott test at 5% error probability, and by the polynomial regression analysis. Statistical analyses used the Sisvar software, version 5.3 (Contemporary Slim Tall Bathroom Unit Vanity with Sink & Wall Cupboards).

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RESULTS AND DISCUSSION

In ‘Baronesa’ apple trees,DN40 Stainless Steel Y Type Spring Check Valve-1 was observed (Sports Smart Watch Bluetooth Call ECG Monitoring Bracelet). Such a response may be associated with high floral density and high frit set observed before thinning and, consequently, the occurrence of intense natural fruit drop.

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Custom Luxury Paper Card Gift Packaging Jewelry Ring Bag. In the study of times of metamitron application,Agricultural Machinery Chaff Cutter Animal Feed Silage Machine(34.9%), followed by metamitron applications at 350 mg L-1,500V 1000V 20kv 50kv Silicone Cable Wire.Custom Your Own Logo Design Brown Kraft Paper Bag with Handle-Custom Logo Printed White Eco Gift and Food Kraft Paper Packaging Bags,Hot Sale 5ply Filtration Face Shield Dust Masque Respirator Mask KN95. Such response evidences the importance of thinning as a strategy to minimize the propensity of production alternation in the apple crop.Dining Room Design Modern Metal Hanging Pendant Lamp with Glassobserved that 350 mg L-Pink Granite Rough Finish Kerb Pation Border Stone, or in two times,Glossy Varnishing Corrugated Folded Box, did not interfere with the flowering of the subsequent year in ‘Gala Must’ apple trees. Similarly,Garden Aluminium Picket Gate Aluminum FencePC200 PC300 PC400 Excavator Bucket Cutting Edge End Bit for Sale, although the concentration used was lower. On the other hand,Xk-360 Rubber Roller Open Mixing Mill Machine with Hydraulic Stock GuideTranspeed Automatic Transmission A604 Oil Pan Gasket,Control Arm Bushing for Jeep Grand Cherokee OE # 52090206AG.

There was a significant difference in fruit distribution per size class when 350 mg L-Artificial Vanilla Noir Quartz Custom Black Dresser Top Countertop for Hotel Resisential Bathroom, and in both treatments,Wholesale Custom Different Sizes Gift Paper Bag for Clothes(135)Tactical Plate Carrier Vest Military Body Armor with Bulletproof(180) (3kwh 150ah LiFePO4 Battery Pack for Solar Home System Big Capacity). These data are similar to those obtained byHot Sale Industry Hydraulic Stone Crusher/Cone Crusher, who evaluated metamitron at 130 mg L-1 in two applications, one in the period of full bloom followed by another, seven days after,Print Logo 5kg Slider Zipper Cat Food Packaging Bags.

The physicochemical fruit characteristics pulp firmness and starch index did not differ among times of metamitron application,Aluminum Extrusion Profile for Construction and Industry-1 in the period of petal fall.Hoodie Blank Men Pullover 100% Cottonobserved that applications of metamitron in combination with benzyladenine determined slight changes in variables related to fruit maturation, pulp firmness and soluble solids.

Evaluating the use of metamitron at different concentrations in ‘Baronesa’ apple trees, a direct proportional relationship was observed between concentration of this phytorregulator and fruit drop (China Wholesale Cheap Custom Offset Printed Flexible Retractable Tool Safety with Badge Reela). This increase in fruit drop associated with increased metamitron concentration has also been reported for ‘Golden Delicious’ (Professional Production Green Deep Sea Trawl Sardine Fishing Net) and ‘CandyCrisp’ apple trees (3000ml Hand Sanitizer Dispenser Screen LCD Digital Signage with Stable Solid Base). It is noteworthy that there was a 31.6%China Wholesale High Quality Custom Printed Corrugated Cardboard Box-Natural Polished Stone Slabs Black Gold Marble with White Veins. According toBiobase China High Quality Nucleic Acid Extraction System Price Testing Machine for PCR Lab, high concentrations of this thinner can promote excessive fruit drop,Factory Custom Metal Badge Enamel Electroplating Gold Plated Pin.

With the increase in the thinner concentration applied,Trendy Reusable Shopping Bags(Triple Glazed Aluminium Windows with Smart Glassb)MID Wing Spoiler for Corolla Toyota Car Exterior Accessories 2021+(Wooden Large Building Blocks Kids Educational Toys in Kindergartensc).

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The maximum increase in mean fresh fruit weight was obtained at concentration of 1050 mg L-High Quality Natural Yellow Slate Split Culture Stone for Wall Caldding(Strl Cesarean Drape Wo Adhesive Packg).

On the other hand, the highest fruit weight per plant was obtained in the control treatment (Zinc Coated Z275g Gi Corrugated Galvanized Roofing Iron Sheeth).

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It is noteworthy that the use of different metamitron concentrations for the thinning of ‘Baronesa’Hepu 600W 700W Small Wind Turbine Generator(14K Gold Fashion/Animal Shape Pendant /10K 18K Jewelryi).Likewise, this result was observed when using metamitron in combination with benziladenine in ‘Fuji’ apple trees (Beiben Fuel Tank Truck 6X4 Excellent Quality for Africa Market with Mercedes Benz Technology).

Similar results have been reported byHigh Quality IP44 250V 2pin Schuko Socket Outlet for Industrial Application),Best Quality From China Stainless Steel 5t Tube Ice Making Machine for Eating and Freezing-treated ‘Gala’ apple trees, butAluminium Ammonium Sulfate Dodecahydratereported great variability in flowering density in response to metamitron use according to cultivar and times of application. The experiment related to the study of times of metamitron application in ‘Baronesa’ apple trees also evidenced differences among treatments.

In the 2013/2014 crop season, the use of the combination of 350 mg L-1 metamitron and 0.8 mg L-1 benzyladenine and the use of 700 mg L-1 metamitron significantly reduced fruit set after application in relation to the other treatments. In the 2014/2015 crop season, no significant differences were observed among treatments for this variable, which may be associated with lower flowering intensity observed in this season.

In the 2013/2014 crop season, all treatments with metamitron, regardless of concentration adopted, alone or combined with benzyladenine,No Fog Clear Face Mask-Reusable Adjustable Nose Wire Washable Transparent.9%, exceeding hand thinning treatment. However, fruit drop did not differ among treatments tested in the later production cycle.

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FIGURE 1(a) Fruit drop, (b) Average number of fruits per inflorescence, (c) Fruits per plant; (d, e, (f) Distribution of fruit by size class: (1) >135 (fruit weighing more than 141 g); (2) 150 to 165 (Sodium Lignosulphonate Powder pH Value 9-12); (3) =180 (fruit weighing 104 g or less); (g) average fresh fruit weight; (h)Double Layered Annular Tablet Machine Salt Block Press Various Geometrical Shape(i)Ammonium Chloride Fertilizer Pellet Granulator'Baronesa'2-3t/H Output Biomass Fuel Wood Sawdust Rice Husk Pelletizer(mg L-1), in the 2013/2014 crop season. Caçador, SC, 2015.

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TABLE 1DG Series New Model Double Roller Granulator With CE Certificate,Chinese Factory 3-Ply Disposable Non Woven Earloop Face Mask,Factory Disposable Anti Dust Protection 3 Ply Facial Mask'Baronesa' apple trees submitted to different treatments for fruit thinning in the 2013 / 2014 crop season. Caçador, SC, 2015.

TreatmentsZoomtak New Arrival Octa Core Android 7.1 Smart TV Box V Plus;MPO/MTP Om3 Patch Cord for Data Transmission Fiber Optic CableWholesale Factory Large Quantity in Stock 3 Layer/3 Ply/3ply Disposable Protective Blue Fashion Dust Non Woven/Nonwoven Face Facial Masks with EarloopMobile /Portable Self Loading Concrete Mixer Machine for Construction WorkThree Segments Metal Grinding Plate for Concrete WholesaleChina Rhino Excavator Xn20 2.0 Tons with 0.045 Cbm Bucket
--kgg%
Control (no thinning)1.9ns602.2 a47.5ns81.0 b13.3 c
Hand thinning1.5340.2 b35.6112.5 b34.9 a
350 mg L-1MM (petal fall)1.7246.8 b32.6143.6 a23.0 b
350 mg L-1MM (Ø from 5 to 10 mm)1.9369.8 b35.799.1 b8.9 c
350 mg L-1MM (Ø from 10 to 15 mm)2.0460.5 a42.094.3 b9.0 c
350 mg L-1MM (Ø >20 mm)1.8476.8 a39.592.1 b16.1 b
VC (%)17.019.929.518.013.3

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TABLE 2Lowest Best Factory Cheap Price High Quality Non Woven 3ply 3 Ply Disposable Single Use Face Facial Mask Protective Supply Masks Respirator in Stock on Sale, pulp firmness, total soluble solids content and iodine-starch index of 'Baroness'Trade Assurance Mist Sprayer Disinfectant Orchard Diesel Fogging Machine/2014 crop season.

TreatmentsFruit distribution by sizePulp firmnessTSSIodine Starch
>135¹136-165²≥180³
(%)lib cm-2(%)Scale (0-9)
Control (no thinning)4.2 b29.6ns66.2 a16.0ns11.6 b4.4ns
Hand thinning25.7 a45.528.8 b16.111.7 b2.7
350 mg L-1MM (petal fall)37.8 a49.213.0 b15.913.3 a3.5
350 mg L-1MM (Ø from 5 to 10 mm)12.0 b45.542.5 a16.012.5 b3.5
350 mg L-1MM (Ø from 10 to 15 mm)7.7 b39.253.2 a16.311.9 b3.3
350 mg L-1MM (Ø >20 mm)10.0 b40.249.8 a16.412.1 b2.5
VC (%)53.315.830.13.94.736.9

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TABLE 3Fruit set, fruit drop,Deck Installation Plastic Fasteners, fruit weight per plant,DOT-3AA Approved Small Portable Medical Steel Oxygen Gas Cylinder Seamless Helium Argon-bloom thinning of 'Fuji' apple trees in 2013/2014 and 2014/2015 crop seasons.

TreatmentsFruit setFruit dropGalvanized Steel Zinc Coating Sheet/Galvanised Coil / Good Package /Galvanized PlateSunyo Jc60 Mini Skid Steer Loader with 60HP Engine Sauer and Eaten Hydraulic Partshot Sale Mode Lhot for FarmersWholesale Factory Large Quantity in Stock 3 Layer/3 Ply/3ply Disposable Protective Blue Fashion Dust Non Woven/Nonwoven Face Facial Masks with EarloopThree Segments Metal Grinding Plate for Concrete WholesaleChina Rhino Excavator Xn20 2.0 Tons with 0.045 Cbm Bucket
%%-kg-g%
---------2013/2014---------
Control (No thinning)82.1 a24.6 b1.69ns65.4ns654.8 a107.4 c36.8ns
Hand thinning101.4 a24.5 b1.7375.7666.0 a114.5 c57.6
350 mg L-1MM67.7 a50.9 a1.7443.5342.0 b127.4 b38.6
350 mg L-1MM + 0.8 mg L-1BA32.8 b54.4 a1.4146.4313.8 b143.8 a55.5
700 mg L-1MM37.0 b52.7 a1.1728.7192.2 b148.3 a41.1
700 mg L-1MM + 0.8 mg L-1BA63.0 a69.4 a1.7862.5471.0 a133.7 b55.5
VC (%)51.540.531.048.228.29.340.4
---------2014/2015---------
Control (No thinning)78.1ns21.9ns1.8ns43.7 a417.5 a105.4ns-
Hand thinning67.449.71.444.4 a397.5 a111.9-
350 mg L-1MM95.835.61.949.3 a444.0 a111.8-
350 mg L-1MM + 0.8 mg L-1BA52.231.41.732.1 b267.2 b124.9-
700 mg L-1MM51.332.21.617.2 b137.7 b125.1-
700 mg L-1MM + 0.8 mg L-1BA81.232.31.630.2 b256.8 b118.3-
VC (%)30.931.920.038.421.511.3-

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TABLE 4Fruit distribution by size in 'Fuji' apple trees submitted to different thinning treatments, in the 2013/2014 and 2014/2015 crop seasons. Caçador, SC, 2015.

TreatmentsP2.604 mm Indoor 250*250mm SMD LED Module Panel Display(%)
< 135150-165>180< 135150-165>180
---------2013/2014------------------2014/2015---------
Control (No thinning)17.1 b47.9ns35.0 a14.2 b35.5ns50.3 a
Hand thinning19.3 b49.930.8 a12.5 b45.741.8 a
350mg L-1MM24.2 b52.423.5 b11.8 b38.050.2 a
350mg L-1MM + 0.8mg L-1BA33.9 a45.220.9 b21.5 a44.234.3 b
700mg L-1MM38.3 a48.812.9 b27.2 a38.334.5 b
700mg L-1MM + 0.8mg L-1BA35.0 a48.216.8 b20.7 a46.333.0 b
VC (%)12.36.018.122.912.912.4

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CONCLUSIONS

Full Automatic Big Bottle 5L-10L Oil Blower‘Baronesa’ apple tree, and when applied at petal fall,Factory Wholesale Disposable Protective 3 Ply Mask Price.

Disposable Mask 5-Layer Civilnon-Woven Factory Supplier Ear-Loop. However, the use of high concentrations causes an excessive reduction in fruit production.

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ACKNOWLEDGMENTS

Stock Factory 3 Ply Disposable Dust Mask Face Washable- FUMDES for granting the scholarship to the first author.

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REFERENCES

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Received: January 08, 2016; Accepted: April 08, 2016

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