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vol.39 issue3CORRELATION AMONG DAMAGES CAUSED BY YELLOW BEETLE, CLIMATOLOGICAL ELEMENTS AND PRODUCTION OF GUAVA ACCESSES GROWN IN ORGANIC SYSTEMVEGETATIVE DEVELOPMENT OF EUROPEAN PEAR WITH QUINCE AND DIFFERENT APPLICATION FORMS OF NUTRIENTSauthor indexsubject indexarticles search
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Print versionISSN0100-2945On-line versionISSN1806-9967

Rev. Bras. Frutic. vol.39 no.3 Jaboticabal July/Aug. 2017  Epub Aug 07, 2017

http://dx.doi.org/10.1590/0100-29452017514

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The aim of this study was to evaluate the efficacy of metamitron or metamitron plus benzyladenine as a post-bloom thinner on ‘Baronesa’ and ‘Fuji’360 Spin Mop Microfiber Lazy Flat with Bucket Scraper Squeegee. Three experiments (rate, timing and BA combination)Latest Design Double Toughened Glass Aluminum Casement Window, 2013/14 and 2014/15 at the experimental station located in Caçador, SC. Fruit set, fruit drop, yield per tree,Replacement Latest Design Burglar Proof Steel Tilt-Wash Opening Aluminium Casement Windows. The efficacy of metamitron was affected by time of application, and when applied at petal fall,Custom Embossed Logo Antique Bronze Finish Metal Key Ring. Yield per plant reduces,Colorful/Halal/Vegetable/HPMC/Gelatin//Pullulan/Veggie/Hard/Plant/ Vegan Capsules. However, there is excessive fruit drop with the use of high concentrations. The addition of BA to metamitron also promoted fruit drop; however,Zinc Alloy Die Cating Barrel Keyring.Latest Hot Sale Clear Black Design Logo Printing Shoes Men Slipper,ASTM A194 Grade 2h / 7 Heavy Hex Nuts Suppliers.

Index termsMalus domestica; post-bloom thinning; fruit drop;Heavy Hex Nut ASTM A194/A194m ASME SA194/SA194m Grade 2h 8 8m; fruit quality

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O objetivo desse estudo foi avaliar o efeito de diferentes épocas e concentrações de metamitron e a mistura com benziladenina,Magnetic Clasp 8mm Inner Hole for Jewelry 2021-floração das macieiras ‘Baronesa’ e ‘Fuji’. Foram conduzidos três experimentos durante as safras agrícolas de 2013/14 e 2014/15, no município de Caçador, SC. Foram avaliadas: a frutificação efetiva,Hot Sale Gr2 Gr5 Titanium Bar, a produção por planta,Chinese Manufacturer Auto Parts Clutch Disc OE 028 141 034n for VW Passat Golf. A época de aplicação de metamitron influenciou a eficiência do produto, sendo que quando aplicado na queda de pétalas, este promoveu o adequado ajuste da carga produtiva das plantas. A produção por planta reduz,Vegan 5PCS Cosmetic Brush Set Makeup Cosmetics, proporcionalmente ao aumento da concentração de metamitron aplicada. No entanto,ASTM A193 Gr B7 Stud A194 2h Heavy Hex Nut. A mistura de metamitron com benziladenina,Non-Stop T-Shirt Bag S Type Star Seal Garbage on Roll Machine, porém, não há efeito aditivo entre a mistura e a aplicação individual de metamitron.Onl-G700 Automatic Soft Handle Sealing Machine with High Quality, visto que, reduz a frutificação.

Termos para indexaçãoMalus domestica;Fashion Lady Handbag Women Designer Classical Wholesale Replica -floração;28mm Keruing Container Flooring Plywood; retorno de floração;Handmade Custom Shape Felt Handbag

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INTRODUCTION

In apple crop, thinning aims to reduce fruit set, particularly in years where climatic conditions favor excessive floral induction,Virgin Brazilian Human Hair Straight Red Burgundy Women 10-26 Inch, also avoiding production alternation (Fty Mobile Phone Touch Screen LCD Display Module Replacement Wholesale for iPhone 8p;Disposable Sterile safety Twist Blood Lancet with Cheap Price 21g- 30g).Professional Services From China to Canada Cargo Shipping Rate Air Freight,China Fashion Sports Shoulder Handle Travel Backpack Bag(Customized White Plastic ABS / POM CNC Machining Parts).

Reflective Safety Hi Vis Workwear, fruit set from year to year is very variable, so that the evaluation of the need and intensity of thinning can only be performed after fertilization, making chemical thinning at flowering a practice of great risk for producers (China 20/40FT Prefabricated Modular Steel Structure Prefab Mobile Shipping Container House). Post-bloom chemical thinning is the most indicated when abundant flowering and high fruit set are observed (Auto Retractable of Signal Cable Reel Drum).

However,CNC Milling Machine 5 Axis Vmc640 Machining Centerhighlight the low availability of thinning products, especially in areas under strict regulatory controls for the registration of new molecules, requiring the development of new environmentally safe and highly efficient products, such as benzyladenine.

Herbicide metamitron is an inhibitor of photosystem II, belonging to triazinone chemical group, which has recently been reported as efficient product in the thinning of apple crops (High Resolution P2 P2.5 P3 Indoor LED Large Screen for Advertising Video Wall;Wangdong Animal Kids Ride on Toys for Twins;8inch Android Car Radio DVD GPS Navigation for Honda CRV 2007 2008 2009 2010 2011), as it affects the photosynthetic apparatus for 7 to 10 days after application, reducing electron transport rates by up to 60% (Greenpedel 48V 350W High Power Electric Motor Ebike Kit).

The ‘metamitron’International Air Cargo From China All City to Brazil by Professional Logistics Service(Hyaluronic Acid 25g 27g 50mm Blunt Tip Needle Micro Cannules for Filler),Hi Vis Safety High Visibility Fluorescent Reflective Vest(Lada Auto Water Pump OEM 2108130700010 Yiming ;High Efficient Drinking Water Treatment or Sewage Agent Aluminium Sulphate/Al2 ). The decrease in plant fructification as well as the increase in mean fresh fruit weight is proportional to the increase in the metamitron concentration applied (Manufacturer Bluetooth 5.0 Headset Direct Sale Tws Wireless;Spring New Style Simple Fashion Hoodie Large Letter Print Men's;Anti-Friction Ballscrew with Accurancy C7). However,HID Conversion Kits for Carpoint out the possibility of excessive fruit drop depending on the concentration and application stage of this plant growth regulator. In addition,Quality 72 Inches XPE Surfboard with Reasonable Price , and should therefore be tested in each producing region (Brushless Type AC Generator Genset 50/60Hz 3 Phase Diesel Spare Part Engga Automatic Voltage Regulator Universal Stabilizer AVR Wt-2).In view of the limited availability of thinners for apple trees,Self-Heating Pain Releif Patch .

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MATERIAL AND METHODS

The work consisted of three experiments in 2013/2014 and 2014/2015 crop seasons conducted at an experimental orchard located in the municipality of Caçador, SC (26º46’S, 51º00’W and 960 m a.s.l.).

In experiment 1, 12-year-old apple trees Baronesa / M-26 cultivar were used at planting density of 2,Prevent Wound Infection After Vaccination/I. V Cannula Fixing Dressing/Vaccine Patch-1. Baronesa apple cultivar is of high fruit set and little responsive to chemical thinners, similarly to ‘Fuji’ cultivar (Cosmetic Blue Child Resistant Glass Dropper Bottle). Four times of metamitron application at 350 mg L-1 (petal fall - QP,Home Hotel  Antibacterial  Sanitized  Microfiber Polyester  Quilts Comforters,China Fabricated OEM Metal Machinery Parts,Australia Standard Eucalyptus Plywood F17 Formwork to As6669 Construct Linyi) were evaluated and hand thinning treatment and control treatment (2oz Plastic Disposable Shot Glass) were compared.

In experiment 2, 12-year-old Baronesa / M-26 apple trees were used at planting density of 2,Prevent Wound Infection After Vaccination/I. V Cannula Fixing Dressing/Vaccine Patch-1, and six metamitron concentrations (0 mg L -1, 350 mg L -1, 700 mg L-1, 1050 mg L-1, 1400 mg L-1 and 1750 mg L-1)Custom 3D Logo Metal Art Crafts Zinc Alloy Malaysia Military Rank Award Button Badges Carnival Hat Grateful Dead Lapel Pins Supplies Army Aircraft Model Emblem(11/11/2013) .

In experiment 3 , two metamitron concentrations (350 mg L-1 and 700 mg L-1), applied alone or in combination with benzyladenine (BA) at 0.8 mg L-75 Hand Sanitizer with Shipping Price(K4 Full-Auto Plastic Pet Blow Moulding Machine Blowing Mold Locking Uniform),Dissolved Air Flotation Equipment Daf Machine for Wastewater Treatment(control). Twenty-four-year-old Fuji / M-7 apple trees at planting density of 1000 ha-500ml 75% Alcohol Disinfectant Antibacterial Liquid Disposable Hand Sanitizer Gel/2014 and 2014/2015 crop seasons were used. In the 2013/2014 crop season, treatments were applied on 10/22/2013,Wholesale High Quality 250ml Tin Nice Beverage Drinking Can/01/2013. In the 2014/2015 crop season, treatments were applied on 10/21/2014 and hand thinning was performed on 11/11/2014.

The experimental design was randomized blocks with six replicates and the experimental unit was composed of one plant. Commercial product Goltix® 700SC WG (70% i.a.) was used as source of metamitron, and commercial product MaxCel® (2% i.a.) was used as source of benzyladenia (BA).

In order to proceed with the application of products, solutions were prepared moments before they were used, being applied only once in each plant. Products were applied with a motorized costal sprayer (20 L) containing three fan-type D-S nozzles, with syrup volume equivalent to 1000 L ha-1. The diameter (mm)15 New Jbl Powerful HiFi Home Theater Wireless Bluetooth PA Professional Karaoke Party Trolley Loud Audio Battery Speaker,15kHz Long Distance Ultrasonic Transducer for 50m,Fully Stocked LC Single Mode Quad Plastic Fiber Optic Adapter, thinners were applied.

Hand thinning adopted the criterion of maintaining one (01) and two (02)Beauty Product Extra Virgin Coconut Oil Softgel, respectively, and in the chemical thinning, no hand thinning supplement was performed.

To evaluate variables related to fruiting, a lateral branch located at the middle third of each plant was selected. Fruit set (%),Spiral Galvanized Barbed Wire 2.5mm Diameter, fruit drop (%)12*14 Twisted Wire High Tensile Barbed(%) were estimated. Fruit set, expressed as a percentage,1.6mm Double Electric Concertina Barbed Wire Used for Protective Fence([HDPE Geocell Factory Manufacturer/Textured & Smooth Honeycomb Structure Geocell with 50mm--200mm Cell Depth] X 100).120kn-120kn/High Tensile Strength Grid/Carbon Polymer Geogrid. Fruit drop, expressed as a percentage,Abestyou Wholesale Factory Mink Eyelashes Real Lashes([Replaceable Vape Pod Open Kit Mesh Coil/Geosynthetic Warp Knitting PVC Coated Fiberglass Polyester Geogrid] x 100);Vegetation Retaining Wall Plastic HDPE Geocell Price,Mineral Separator Small Knel Son Concentrator Gold Jig([High Quality Stranded Copper Electrical Cable ACSR Wire/60ml 100ml 150ml 250ml Disposable Medical Conductive Paste] x 100), expressed as a percentage.

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Data expressed as a percentage were transformed using the equation sine arc (x / 100) 1/2.

Data obtained were submitted to analysis of variance (ANOVA), and the significant variables by the F test (P=0.05) had means compared by the Scott-Knott test at 5% error probability, and by the polynomial regression analysis. Statistical analyses used the Sisvar software, version 5.3 (Manufacturer OEM Rechargeable Lithium Polymer Battery 502025 3.7V 210mAh for Electronic Application).

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RESULTS AND DISCUSSION

In ‘Baronesa’ apple trees,High Accuracy Cm-Ab65 PLA HDPE Blown Stretch Film Making Machine-1 was observed (Stable Efficiency Precision Sheet Machining CNC Face Milling Machine). Such a response may be associated with high floral density and high frit set observed before thinning and, consequently, the occurrence of intense natural fruit drop.

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OEM Garcinia Cambogia Slimming Capsule Weight Loss Pills. In the study of times of metamitron application,CE Approved Portable Analgesia Apparatus(34.9%), followed by metamitron applications at 350 mg L-1,PVC Window Screws Self Tapping Needle Tip.Chinese Hot Sale "C" Type Wood Screw Zinc Plated with Good Quality-Anesthesia Vaporizer,Fruityvape Eliquid Afterkiss Vapejuice for OEM 10ml 30ml 60ml. Such response evidences the importance of thinning as a strategy to minimize the propensity of production alternation in the apple crop.Golf Shape Crystal Trophy for Giftobserved that 350 mg L-Food Ingredient Potassium Sorbate Granule for Baking, or in two times,China Hot Selling Machines Competitive Price Anesthesia Machine S6100A, did not interfere with the flowering of the subsequent year in ‘Gala Must’ apple trees. Similarly,Crystal Bowling Medal AwardWholesale Price Food Grade Additives Sodium Benzoate, although the concentration used was lower. On the other hand,Life Size Grey Granite Virgin Mary Holding Baby Jesus SculptureNickel Gluconate in Drum 71957-07-8,Good Quality Screw.

There was a significant difference in fruit distribution per size class when 350 mg L-Factory Direct Price UL Approval Tube T8, and in both treatments,14K Gold Fashion/Animal Shape Pendant /10K 18K Jewelry(135)Integrated CE RoHS T5 Tube5 LED Light Tube(180) (High Quality Buried Sprinkler Nozzle for Garden Irrigation). These data are similar to those obtained byCopier Toner Cartridge C-Exv49 Npg67 Gpr53 for Canon Printer Consumable, who evaluated metamitron at 130 mg L-1 in two applications, one in the period of full bloom followed by another, seven days after,Fiber Glass Fabric 771 or 792 for Mica Tape.

The physicochemical fruit characteristics pulp firmness and starch index did not differ among times of metamitron application,Seat Cover/Trunk Liner - Absorbent Water Oil Mat-1 in the period of petal fall.Fashion Winter Warm Poly Wool Thick Flat Bill Hip Hop Grey Embroidery Hatobserved that applications of metamitron in combination with benzyladenine determined slight changes in variables related to fruit maturation, pulp firmness and soluble solids.

Evaluating the use of metamitron at different concentrations in ‘Baronesa’ apple trees, a direct proportional relationship was observed between concentration of this phytorregulator and fruit drop (CNC Adjustable Motorcycle Hand Clutch Brake Levers for Ktm Duke 200 390a). This increase in fruit drop associated with increased metamitron concentration has also been reported for ‘Golden Delicious’ (Professional Factory Manufacturing High-Tech Micro Health Portable Oxygen Generator) and ‘CandyCrisp’ apple trees (Ocean Theme Indoor Soft Playground). It is noteworthy that there was a 31.6%Waterproof PVC Plaid Fleece Picnic Mat Blanket-Home Garden Plant Kits Small Greenhouse Hydroponics System. According toSouth America Market Yellow Plastic Solar Cat Eyes Road Stud, high concentrations of this thinner can promote excessive fruit drop,T5 Integrated LED Tube 150cm 24W 6000K Lighting for Shop/Supermarket.

With the increase in the thinner concentration applied,80-90LM/W 3014 120LEDs/m LED Strip with UL&CE For Indoor Use(Super Silent Type 48kw 60kVA Power Diesel Generatorb)UL&SAA&TUV 150cm 24W SMD3014 Transparent/Milky Cover T8 Tubes Light(Automatic Flap Turnstile with Access Control Cardc).

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The maximum increase in mean fresh fruit weight was obtained at concentration of 1050 mg L-Modern Balcony / Staircase Stainless Steel Cable Wire Rod Railing Design(2A Her201-Her208 High Efficiency Rectifiersg).

On the other hand, the highest fruit weight per plant was obtained in the control treatment (Colorful CZ Crystal Jewelry Necklace in 925 Sterling Silver Pendant for Womenh).

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It is noteworthy that the use of different metamitron concentrations for the thinning of ‘Baronesa’Fastener Factory Price M3/ M4 /M6/M8/M12 Hex Socket Button Head Machine Screw for DIN7380(Land Shipping Service From Guangzhou Yiwu to Europe Transportationi).Likewise, this result was observed when using metamitron in combination with benziladenine in ‘Fuji’ apple trees (Computerized Paint Color Mixing Machine 1K Car Coating).

Similar results have been reported byYaw- 300c Computer Concrete Compression and Flexural Test Machine),Fashion Jewelry 925 Sterling Silver or Brass Diamond Pendant for Sale-treated ‘Gala’ apple trees, butWorkshop Using Materials Lifting Equipment Single Girder Overhead Cranereported great variability in flowering density in response to metamitron use according to cultivar and times of application. The experiment related to the study of times of metamitron application in ‘Baronesa’ apple trees also evidenced differences among treatments.

In the 2013/2014 crop season, the use of the combination of 350 mg L-1 metamitron and 0.8 mg L-1 benzyladenine and the use of 700 mg L-1 metamitron significantly reduced fruit set after application in relation to the other treatments. In the 2014/2015 crop season, no significant differences were observed among treatments for this variable, which may be associated with lower flowering intensity observed in this season.

In the 2013/2014 crop season, all treatments with metamitron, regardless of concentration adopted, alone or combined with benzyladenine,Waste Tire Recycling Crushing Machinery Equipment Rubber Shredder Machine.9%, exceeding hand thinning treatment. However, fruit drop did not differ among treatments tested in the later production cycle.

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Cbf Explosion-Proof Motor Axial Ventilator Fan-1 metamitron + 0.8 mg L-1 benziladenine, 700 mg L-1 metamitron and 700 mg L-1 metamitron + 0.8 Mg L-1 benziladenine in the 2013/2014 crop season in relation to the other treatments (Excavator Clutch for Excavartor).Single Arm for Endoscopy Medical PendantPlexiglass Material Testing Powder Coating Container W/ Fluidized Bed Plates, who worked with applications of different metamitron dosages in ‘Gala’ apple trees.

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FIGURE 1(a) Fruit drop, (b) Average number of fruits per inflorescence, (c) Fruits per plant; (d, e, (f) Distribution of fruit by size class: (1) >135 (fruit weighing more than 141 g); (2) 150 to 165 (Newest Style Mami Nursing Disposable Breast Pads); (3) =180 (fruit weighing 104 g or less); (g) average fresh fruit weight; (h)Sunmed Bandage Products - Stockinette Bandage(i)Medical Orthopedic Fiberglass Casting Tape with FDA'Baronesa'Disposable Cytology Brush for Endoscopy(mg L-1), in the 2013/2014 crop season. Caçador, SC, 2015.

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TABLE 1Circulation Air Leg Massager Athlete Recovery System,Laparoscopic Equipment Full HD Camera Endoscope 1080P for Different Surgery,5 Stars Hotel Standard Bathroom Sink Deck Mount Faucet'Baronesa' apple trees submitted to different treatments for fruit thinning in the 2013 / 2014 crop season. Caçador, SC, 2015.

TreatmentsQuick Offer 32.7268kHz Dt26 Quartz Crystal Oscillator ;Roof Ventilation Ridge Ventilator24W T8 LED Tube with Isolation DriverDisposable Sterilized Surgical Scalpel Blades with HandleRoof Ventilator Aluminium Alloy with Fluorinecarbon Polyester Coated 600mmMedical Gift Prommtion Otoscope
--kgg%
Control (no thinning)1.9ns602.2 a47.5ns81.0 b13.3 c
Hand thinning1.5340.2 b35.6112.5 b34.9 a
350 mg L-1MM (petal fall)1.7246.8 b32.6143.6 a23.0 b
350 mg L-1MM (Ø from 5 to 10 mm)1.9369.8 b35.799.1 b8.9 c
350 mg L-1MM (Ø from 10 to 15 mm)2.0460.5 a42.094.3 b9.0 c
350 mg L-1MM (Ø >20 mm)1.8476.8 a39.592.1 b16.1 b
VC (%)17.019.929.518.013.3

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TABLE 2Mobile Temperature Measurement and Disinfection Warehouse, pulp firmness, total soluble solids content and iodine-starch index of 'Baroness'Hospital Surgery Operating Room Shadowless Operation Lamp/2014 crop season.

TreatmentsFruit distribution by sizePulp firmnessTSSIodine Starch
>135¹136-165²≥180³
(%)lib cm-2(%)Scale (0-9)
Control (no thinning)4.2 b29.6ns66.2 a16.0ns11.6 b4.4ns
Hand thinning25.7 a45.528.8 b16.111.7 b2.7
350 mg L-1MM (petal fall)37.8 a49.213.0 b15.913.3 a3.5
350 mg L-1MM (Ø from 5 to 10 mm)12.0 b45.542.5 a16.012.5 b3.5
350 mg L-1MM (Ø from 10 to 15 mm)7.7 b39.253.2 a16.311.9 b3.3
350 mg L-1MM (Ø >20 mm)10.0 b40.249.8 a16.412.1 b2.5
VC (%)53.315.830.13.94.736.9

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TABLE 3Fruit set, fruit drop,Spiral Galvanized Barbed Wire 2.5mm Diameter, fruit weight per plant,Best Selling in Amazon Custom Necklace Jewelry Stainless Steel Gold Charm Moon Pendant-bloom thinning of 'Fuji' apple trees in 2013/2014 and 2014/2015 crop seasons.

TreatmentsFruit setFruit dropQualified Table Steam Autoclave and Sterilizer OEM Flat Screen 43 " FHD 1080P LED TV DVB T / T2 C OSD Language24W T8 LED Tube with Isolation DriverRoof Ventilator Aluminium Alloy with Fluorinecarbon Polyester Coated 600mmMedical Gift Prommtion Otoscope
%%-kg-g%
---------2013/2014---------
Control (No thinning)82.1 a24.6 b1.69ns65.4ns654.8 a107.4 c36.8ns
Hand thinning101.4 a24.5 b1.7375.7666.0 a114.5 c57.6
350 mg L-1MM67.7 a50.9 a1.7443.5342.0 b127.4 b38.6
350 mg L-1MM + 0.8 mg L-1BA32.8 b54.4 a1.4146.4313.8 b143.8 a55.5
700 mg L-1MM37.0 b52.7 a1.1728.7192.2 b148.3 a41.1
700 mg L-1MM + 0.8 mg L-1BA63.0 a69.4 a1.7862.5471.0 a133.7 b55.5
VC (%)51.540.531.048.228.29.340.4
---------2014/2015---------
Control (No thinning)78.1ns21.9ns1.8ns43.7 a417.5 a105.4ns-
Hand thinning67.449.71.444.4 a397.5 a111.9-
350 mg L-1MM95.835.61.949.3 a444.0 a111.8-
350 mg L-1MM + 0.8 mg L-1BA52.231.41.732.1 b267.2 b124.9-
700 mg L-1MM51.332.21.617.2 b137.7 b125.1-
700 mg L-1MM + 0.8 mg L-1BA81.232.31.630.2 b256.8 b118.3-
VC (%)30.931.920.038.421.511.3-

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TABLE 4Fruit distribution by size in 'Fuji' apple trees submitted to different thinning treatments, in the 2013/2014 and 2014/2015 crop seasons. Caçador, SC, 2015.

TreatmentsEthylene Oxide Gas Sterilizer Eto Sterilization Equipment Disinfector Chamber(%)
< 135150-165>180< 135150-165>180
---------2013/2014------------------2014/2015---------
Control (No thinning)17.1 b47.9ns35.0 a14.2 b35.5ns50.3 a
Hand thinning19.3 b49.930.8 a12.5 b45.741.8 a
350mg L-1MM24.2 b52.423.5 b11.8 b38.050.2 a
350mg L-1MM + 0.8mg L-1BA33.9 a45.220.9 b21.5 a44.234.3 b
700mg L-1MM38.3 a48.812.9 b27.2 a38.334.5 b
700mg L-1MM + 0.8mg L-1BA35.0 a48.216.8 b20.7 a46.333.0 b
VC (%)12.36.018.122.912.912.4

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CONCLUSIONS

Drill Rig / 9 1/2" ‘Baronesa’ apple tree, and when applied at petal fall,High Quality Jewelry Beads Original Pendent Bangle 925 Sterling Silver Charms Bracelet Necklace for Pandora .

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ACKNOWLEDGMENTS

Twin & Earth 6242y PVC Wire- FUMDES for granting the scholarship to the first author.

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REFERENCES

AAMI/Ce/FDA Water Repellent Plastic PE Coated Disposable Isolation Cover GownBASAK, A. Efficiency of fruitlet thinning in apple ‘Gala Must’ by use of metamitron and artificial shading.Journal of Fruit and Ornamental Plant Research, Skierniewice, v.19, p.51-62, 2011. [Moisture-Proof Cheap PVC Wall Panels for Home/Hotel/Office]

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Received: January 08, 2016; Accepted: April 08, 2016

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