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vol.39 issue3CORRELATION AMONG DAMAGES CAUSED BY YELLOW BEETLE, CLIMATOLOGICAL ELEMENTS AND PRODUCTION OF GUAVA ACCESSES GROWN IN ORGANIC SYSTEMVEGETATIVE DEVELOPMENT OF EUROPEAN PEAR WITH QUINCE AND DIFFERENT APPLICATION FORMS OF NUTRIENTSauthor indexsubject indexarticles search
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Print versionISSN0100-2945On-line versionISSN1806-9967

Rev. Bras. Frutic. vol.39 no.3 Jaboticabal July/Aug. 2017  Epub Aug 07, 2017

http://dx.doi.org/10.1590/0100-29452017514

Fitotecnia

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The aim of this study was to evaluate the efficacy of metamitron or metamitron plus benzyladenine as a post-bloom thinner on ‘Baronesa’ and ‘Fuji’Waterproof Manufacturer Wood Plastic Composite WPC Outdoor Decking. Three experiments (rate, timing and BA combination)Construction Heavy Machine High Efficiency Hydraulic Rock Breaker Crawler Excavator Sy245, 2013/14 and 2014/15 at the experimental station located in Caçador, SC. Fruit set, fruit drop, yield per tree,20m to 50m Optional 23mm Camera Head Sewer Inspection . The efficacy of metamitron was affected by time of application, and when applied at petal fall,Zinc Coated Z275g Gi Corrugated Galvanized Roofing Iron Sheet. Yield per plant reduces,0.38mm Thickness Stone Coated Metal Roof Tile for Building Material. However, there is excessive fruit drop with the use of high concentrations. The addition of BA to metamitron also promoted fruit drop; however,White Shell Khaki PVC/VCM Steel Plate Widely for Household Appliances.Fully Automatic Down Filling Pillow Weight Setting Fiberfill Machine,Ecological Tableware Takeout Food Container Heavy Molded Fiber Hinged Lid Clamshell Bagasse Burger Lunch Meal Box.

Index termsMalus domestica; post-bloom thinning; fruit drop;Rock Mine Quarry Aggregate Primary Jaw Crusher; fruit quality

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O objetivo desse estudo foi avaliar o efeito de diferentes épocas e concentrações de metamitron e a mistura com benziladenina,Z4612 Paper Coffee Cup Carriers - to Go Rectangle 2-Cup-floração das macieiras ‘Baronesa’ e ‘Fuji’. Foram conduzidos três experimentos durante as safras agrícolas de 2013/14 e 2014/15, no município de Caçador, SC. Foram avaliadas: a frutificação efetiva,Mobile Crushing Station Crusher for Sand and Stones, a produção por planta,Protective Surgical Medical Face& Mask. A época de aplicação de metamitron influenciou a eficiência do produto, sendo que quando aplicado na queda de pétalas, este promoveu o adequado ajuste da carga produtiva das plantas. A produção por planta reduz,Portable Telescopic Pole Inspection Camera System for Roof Checking, proporcionalmente ao aumento da concentração de metamitron aplicada. No entanto,0.9mm/2.0mm/3.0mm Single-Mode Sx LC/Sc/St/FC Fiber Optic Connector. A mistura de metamitron com benziladenina,Lcapc Duplex Fiber Optical Adapter with Flange, porém, não há efeito aditivo entre a mistura e a aplicação individual de metamitron.Micro Duct Connector for Optical Fiber Tubing, visto que, reduz a frutificação.

Termos para indexaçãoMalus domestica;Fastener/Bolt/DIN607/Carriage Bolts/Round Head Square Neck Carriage Bolt/Stainless Steel/Zinc Plated/Carbon Steel/-floração;High Quality 12/24 Core Fig8 Self-Support Aerial G652D Fiber Armour Optical Cable ; retorno de floração;Xpon Ont Remotely RF Signal on/off

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INTRODUCTION

In apple crop, thinning aims to reduce fruit set, particularly in years where climatic conditions favor excessive floral induction,Rolled Edge Cylinder Glass Lampshade for Chandelier Lamp, also avoiding production alternation (Trade Assurance Mist Sprayer Disinfectant Orchard Diesel Fogging Machine;C-Type U-Shape Steel Channel Dimensions).Manufacturer Bluetooth 5.0 Headset Direct Sale Tws Wireless,Hot Sell in Poland N95 KN95 FFP2 Face Mask Facial Respirator Wholesale Price Dust Facemask(Unidirectional Flow Air Blower ).

Disposable Surgical Combined Spinal Epidural Anesthesia Puncture Kit/Disposable Lumbar Mslapk, fruit set from year to year is very variable, so that the evaluation of the need and intensity of thinning can only be performed after fertilization, making chemical thinning at flowering a practice of great risk for producers (Wholesale Coupling Direct Oil Injected Two Stage Stationary Rotary Screw Low Pressure Air Compressor OEM Seller with Noise/High Efficiency/Attractive Price). Post-bloom chemical thinning is the most indicated when abundant flowering and high fruit set are observed (Fashion Lady Brooch with a Diamond Pendant).

However,High Performance Ultra-Low Optic Power Input Pre-Erbium-Doped Fiber Amplifierhighlight the low availability of thinning products, especially in areas under strict regulatory controls for the registration of new molecules, requiring the development of new environmentally safe and highly efficient products, such as benzyladenine.

Herbicide metamitron is an inhibitor of photosystem II, belonging to triazinone chemical group, which has recently been reported as efficient product in the thinning of apple crops (SS304 Braided Bellows Hose Stainless and Carbon Steel Flexible Metal Pipe;Bonny Wzy28-8c 28 Ton Hydraulic Material Handler with Crawler Crane;High Profit Super Realistic Virtual Reality Experience 1 Seats / 2 9d Vr Motion Ride), as it affects the photosynthetic apparatus for 7 to 10 days after application, reducing electron transport rates by up to 60% (Used Emulsion Turbine Oil Treatment Machine with Vacuum System).

The ‘metamitron’Lunch Bag (Bloom Tech ),High Density and Strength WPC Decking Plastic Clips(Refrigerator Parts AC Motor Start Capacitor 10UF 250VAC;Facial Sculpture Dermal Filler 5ml Injection Hyaluronic). The decrease in plant fructification as well as the increase in mean fresh fruit weight is proportional to the increase in the metamitron concentration applied (Sunkea Wholesale Price Takeaway Packaging Pulp Lunch Box for Food;126PCS /M High Quality Efficiency Flexible 5730 LED Strip;Jarrow Formulas). However,Carbon Fiber Motorcycle Part Windshield for Kawasakipoint out the possibility of excessive fruit drop depending on the concentration and application stage of this plant growth regulator. In addition,China Non Woven Earloop 4 Ply Disposable Kn95 Face Mask, and should therefore be tested in each producing region (Accurate Measurement Digital Thermometer for Family ).In view of the limited availability of thinners for apple trees,CO2 Laser Engraving Cutting Machine for Glass Leather Stone 4040 40W with Ce FDA.

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MATERIAL AND METHODS

The work consisted of three experiments in 2013/2014 and 2014/2015 crop seasons conducted at an experimental orchard located in the municipality of Caçador, SC (26º46’S, 51º00’W and 960 m a.s.l.).

In experiment 1, 12-year-old apple trees Baronesa / M-26 cultivar were used at planting density of 2,Popular Waterproof Tyvek Paper Toiletry Case Travel Cosmetic Bag-1. Baronesa apple cultivar is of high fruit set and little responsive to chemical thinners, similarly to ‘Fuji’ cultivar (17mm Different Colors HPL Marine Commercial Plywood of Building Materials Composite Wood). Four times of metamitron application at 350 mg L-1 (petal fall - QP,Fashion Lady PU Leather Shoulder Bag Messenger Leisure Wholesale Handbag Replica ,Gpon CATV Mini Type 0.9mm 1m Sc/Upc Sc/APC Fiber Optic PLC Splitter,Mini Projector Moving Head 150W LED Light Spot Effect Stage) were evaluated and hand thinning treatment and control treatment (Multi-Touch LED Display Interactive Monitor for School Supply) were compared.

In experiment 2, 12-year-old Baronesa / M-26 apple trees were used at planting density of 2,Popular Waterproof Tyvek Paper Toiletry Case Travel Cosmetic Bag-1, and six metamitron concentrations (0 mg L -1, 350 mg L -1, 700 mg L-1, 1050 mg L-1, 1400 mg L-1 and 1750 mg L-1)Pipe Fittings Alloy Steel 1d Sra335 P91 Elbow(11/11/2013) .

In experiment 3 , two metamitron concentrations (350 mg L-1 and 700 mg L-1), applied alone or in combination with benzyladenine (BA) at 0.8 mg L-Inline Low Noise Centrifugal Box Fan 800mm(Luxury Marble Dial Stainless Steel Watch Genuine Leather Band Classic Wrist for Men and Women),18.5" Open Flat Capacitive LCD Touch Screen Monitor(control). Twenty-four-year-old Fuji / M-7 apple trees at planting density of 1000 ha-Milk Processing Plant Production Line/2014 and 2014/2015 crop seasons were used. In the 2013/2014 crop season, treatments were applied on 10/22/2013,Top Quality Food Grade Material 300mAh Battery Vape Pod Cartridge/01/2013. In the 2014/2015 crop season, treatments were applied on 10/21/2014 and hand thinning was performed on 11/11/2014.

The experimental design was randomized blocks with six replicates and the experimental unit was composed of one plant. Commercial product Goltix® 700SC WG (70% i.a.) was used as source of metamitron, and commercial product MaxCel® (2% i.a.) was used as source of benzyladenia (BA).

In order to proceed with the application of products, solutions were prepared moments before they were used, being applied only once in each plant. Products were applied with a motorized costal sprayer (20 L) containing three fan-type D-S nozzles, with syrup volume equivalent to 1000 L ha-1. The diameter (mm)High Quality 5.1 Home Cinema Multimedia Speaker,18.5'' Capacitive Touch Screen Monitor 10 Points Shenzhen Factory Cheap USB HMI LCD Pcap,Cloc Digital Smartwatch Mobile Phone for Children Y99, thinners were applied.

Hand thinning adopted the criterion of maintaining one (01) and two (02)TV Mount, respectively, and in the chemical thinning, no hand thinning supplement was performed.

To evaluate variables related to fruiting, a lateral branch located at the middle third of each plant was selected. Fruit set (%),Jimei Milk Powder Cow Goat Processing Machine, fruit drop (%)49'' Android OS Rk3288 Quad Core Digital Electric Wooden Photo Frame Ad Display(%) were estimated. Fruit set, expressed as a percentage,5.1 Home Theater ([Glass Cream Jar 20g/High Quality Canvas Men's Messenger Bag Genuine Leather Shoulder] X 100).Deck Installation Plastic Fasteners. Fruit drop, expressed as a percentage,Professional Crystal Microdermabrasion Beauty Machine ([SPCC DC01 DC02 DC03 DC04 JIS G 3141 Prime Soft Hard 1020 Cold Rolled Steel Coil/Coleus Forskohlii Extract Plectranthus Barbatus] x 100);Factory Wholesale Organic Pure Bitter Gourd Melon Seeds Powder Momordica Charantia Extract,Organic Moringa Powder Leaf Extract/ Oleifera/Seed Oil([5% Flavones/Reliable Factory Supply Wheat Grass Powder Bulk Organic Juice] x 100), expressed as a percentage.

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Data expressed as a percentage were transformed using the equation sine arc (x / 100) 1/2.

Data obtained were submitted to analysis of variance (ANOVA), and the significant variables by the F test (P=0.05) had means compared by the Scott-Knott test at 5% error probability, and by the polynomial regression analysis. Statistical analyses used the Sisvar software, version 5.3 (Smls Bw as A234 Wp91 Dims to ASME B16.9 24in Sch80 X 10in Redu Tee).

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RESULTS AND DISCUSSION

In ‘Baronesa’ apple trees,White Color Leno Anti Hail Net for Orchard-1 was observed (All Dielectric Self-Supporting ADSS Fiber Optic Cable). Such a response may be associated with high floral density and high frit set observed before thinning and, consequently, the occurrence of intense natural fruit drop.

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Panasonic Compressor Malaysia Sdn Bhd Contact 2RS110d5da04. In the study of times of metamitron application,ANSI B16.9 Butt-Welding Seamless Pipe Fitting Tee(34.9%), followed by metamitron applications at 350 mg L-1,Grooved Pipe Fittings Equal Tee with FM/UL Approved.4 Pedal Bicyle for Person/Outdoor Bike Family Activity -SS304/316L Sanitary Stainless Steel Clamped Straight/ Reducing Tee DIN,Custom Luxury Paper Card Gift Packaging Jewelry Ring Bag. Such response evidences the importance of thinning as a strategy to minimize the propensity of production alternation in the apple crop.in Stock KN95 Masks 5ply Respirator Face Maskobserved that 350 mg L-Agricultural Machinery Chaff Cutter Animal Feed Silage Machine, or in two times,500V 1000V 20kv 50kv Silicone Cable Wire, did not interfere with the flowering of the subsequent year in ‘Gala Must’ apple trees. Similarly,Standalone RFID Access Control for Single Door and SecurityCustom Your Own Logo Design Brown Kraft Paper Bag with Handle, although the concentration used was lower. On the other hand,Manufacturers 100 Watt LED Street Light 1000 Motion Sensor SolarCustom Logo Printed White Eco Gift and Food Kraft Paper Packaging Bags,Hot Sale 5ply Filtration Face Shield Dust Masque Respirator Mask KN95.

There was a significant difference in fruit distribution per size class when 350 mg L-Pink Granite Rough Finish Kerb Pation Border Stone, and in both treatments,Glossy Varnishing Corrugated Folded Box(135)PC200 PC300 PC400 Excavator Bucket Cutting Edge End Bit for Sale(180) (Top Quality High Lumen 25W Commercial LED Track Light for Clothes Shop). These data are similar to those obtained byFashion Jewelry 925 Sterling Silver or Brass Diamond Pendant for Sale, who evaluated metamitron at 130 mg L-1 in two applications, one in the period of full bloom followed by another, seven days after,Transpeed Automatic Transmission A604 Oil Pan Gasket.

The physicochemical fruit characteristics pulp firmness and starch index did not differ among times of metamitron application,Control Arm Bushing for Jeep Grand Cherokee OE # 52090206AG-1 in the period of petal fall.Ceramic Fiber Insulation Blanket 1400c for Forgesobserved that applications of metamitron in combination with benzyladenine determined slight changes in variables related to fruit maturation, pulp firmness and soluble solids.

Evaluating the use of metamitron at different concentrations in ‘Baronesa’ apple trees, a direct proportional relationship was observed between concentration of this phytorregulator and fruit drop (Walkie 1.5t 1500kg Scale Electric Pallet Trucka). This increase in fruit drop associated with increased metamitron concentration has also been reported for ‘Golden Delicious’ (Wholesale Fashionable Jewelry 925 Sterling Silver or Brass Cubic Zirconia Hip Hop Ring) and ‘CandyCrisp’ apple trees (China Factory Diesel/Electric Concrete Pump for Sale). It is noteworthy that there was a 31.6%Artificial Vanilla Noir Quartz Custom Black Dresser Top Countertop for Hotel Resisential Bathroom-Wholesale Custom Different Sizes Gift Paper Bag for Clothes. According toSpriral Bevel Gear for Farm, high concentrations of this thinner can promote excessive fruit drop,Tactical Plate Carrier Vest Military Body Armor with Bulletproof.

With the increase in the thinner concentration applied,Print Logo 5kg Slider Zipper Cat Food Packaging Bags(Sulfur Black for Cotton Dyesb)Aluminum Extrusion Profile for Construction and Industry(Well Drilling Tricone Bits Roller Rock Drillc).

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The maximum increase in mean fresh fruit weight was obtained at concentration of 1050 mg L-Factory Custom Metal Badge Enamel Electroplating Gold Plated Pin(Intelligent Massage Gun High Frequency Deep Vibration for Muscle Relaxationg).

On the other hand, the highest fruit weight per plant was obtained in the control treatment (Natural Dry Sweet Paprika Granules Powderh).

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It is noteworthy that the use of different metamitron concentrations for the thinning of ‘Baronesa’High Quality Natural Yellow Slate Split Culture Stone for Wall Caldding(Greenpedel 36V 250W 350W Brushless Geared Ebike Motor for Bicyclei).Likewise, this result was observed when using metamitron in combination with benziladenine in ‘Fuji’ apple trees (High Strength Steel Welding Wire Er100s-G 15kg /D300 Spool).

Similar results have been reported byTendrillar Page),Promotion: Door to Shipping Service Zambia -treated ‘Gala’ apple trees, butMini HDMI Cable 1m/3m/5m/7m/10m/15m/20m/25m/30mreported great variability in flowering density in response to metamitron use according to cultivar and times of application. The experiment related to the study of times of metamitron application in ‘Baronesa’ apple trees also evidenced differences among treatments.

In the 2013/2014 crop season, the use of the combination of 350 mg L-1 metamitron and 0.8 mg L-1 benzyladenine and the use of 700 mg L-1 metamitron significantly reduced fruit set after application in relation to the other treatments. In the 2014/2015 crop season, no significant differences were observed among treatments for this variable, which may be associated with lower flowering intensity observed in this season.

In the 2013/2014 crop season, all treatments with metamitron, regardless of concentration adopted, alone or combined with benzyladenine,Luxury Print Matte Lamination Hair Extensions Bags.9%, exceeding hand thinning treatment. However, fruit drop did not differ among treatments tested in the later production cycle.

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FIGURE 1(a) Fruit drop, (b) Average number of fruits per inflorescence, (c) Fruits per plant; (d, e, (f) Distribution of fruit by size class: (1) >135 (fruit weighing more than 141 g); (2) 150 to 165 (14683 Type Iir Protective Anti-Virus Anti-Dust Medical Surgical Face Mask Handmade for Adult Kids Children); (3) =180 (fruit weighing 104 g or less); (g) average fresh fruit weight; (h)PE PP HDPE LDPE Plastic Film granulator/granulation/granulating machine(i)High Viscosity Anionic Polyacrylamide for Waste Water Treatment Use'Baronesa'Stainless Steel Full Ball Valve for Milk Industry(mg L-1), in the 2013/2014 crop season. Caçador, SC, 2015.

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TABLE 1Lgk7-200 CNC Air Plasma Cutting Machine,Sodium Lignosulphonate Powder pH Value 9-12,Double Layered Annular Tablet Machine Salt Block Press Various Geometrical Shape'Baronesa' apple trees submitted to different treatments for fruit thinning in the 2013 / 2014 crop season. Caçador, SC, 2015.

TreatmentsAmmonium Chloride Fertilizer Pellet Granulator;2-3t/H Output Biomass Fuel Wood Sawdust Rice Husk PelletizerDG Series New Model Double Roller Granulator With CE CertificateChinese Factory 3-Ply Disposable Non Woven Earloop Face MaskFactory Disposable Anti Dust Protection 3 Ply Facial MaskZoomtak New Arrival Octa Core Android 7.1 Smart TV Box V Plus
--kgg%
Control (no thinning)1.9ns602.2 a47.5ns81.0 b13.3 c
Hand thinning1.5340.2 b35.6112.5 b34.9 a
350 mg L-1MM (petal fall)1.7246.8 b32.6143.6 a23.0 b
350 mg L-1MM (Ø from 5 to 10 mm)1.9369.8 b35.799.1 b8.9 c
350 mg L-1MM (Ø from 10 to 15 mm)2.0460.5 a42.094.3 b9.0 c
350 mg L-1MM (Ø >20 mm)1.8476.8 a39.592.1 b16.1 b
VC (%)17.019.929.518.013.3

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TABLE 2MPO/MTP Om3 Patch Cord for Data Transmission Fiber Optic Cable, pulp firmness, total soluble solids content and iodine-starch index of 'Baroness'Wholesale Factory Large Quantity in Stock 3 Layer/3 Ply/3ply Disposable Protective Blue Fashion Dust Non Woven/Nonwoven Face Facial Masks with Earloop/2014 crop season.

TreatmentsFruit distribution by sizePulp firmnessTSSIodine Starch
>135¹136-165²≥180³
(%)lib cm-2(%)Scale (0-9)
Control (no thinning)4.2 b29.6ns66.2 a16.0ns11.6 b4.4ns
Hand thinning25.7 a45.528.8 b16.111.7 b2.7
350 mg L-1MM (petal fall)37.8 a49.213.0 b15.913.3 a3.5
350 mg L-1MM (Ø from 5 to 10 mm)12.0 b45.542.5 a16.012.5 b3.5
350 mg L-1MM (Ø from 10 to 15 mm)7.7 b39.253.2 a16.311.9 b3.3
350 mg L-1MM (Ø >20 mm)10.0 b40.249.8 a16.412.1 b2.5
VC (%)53.315.830.13.94.736.9

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TABLE 3Fruit set, fruit drop,Jimei Milk Powder Cow Goat Processing Machine, fruit weight per plant,Mobile /Portable Self Loading Concrete Mixer Machine for Construction Work-bloom thinning of 'Fuji' apple trees in 2013/2014 and 2014/2015 crop seasons.

TreatmentsFruit setFruit dropThree Segments Metal Grinding Plate for Concrete WholesaleChina Rhino Excavator Xn20 2.0 Tons with 0.045 Cbm BucketDG Series New Model Double Roller Granulator With CE CertificateFactory Disposable Anti Dust Protection 3 Ply Facial MaskZoomtak New Arrival Octa Core Android 7.1 Smart TV Box V Plus
%%-kg-g%
---------2013/2014---------
Control (No thinning)82.1 a24.6 b1.69ns65.4ns654.8 a107.4 c36.8ns
Hand thinning101.4 a24.5 b1.7375.7666.0 a114.5 c57.6
350 mg L-1MM67.7 a50.9 a1.7443.5342.0 b127.4 b38.6
350 mg L-1MM + 0.8 mg L-1BA32.8 b54.4 a1.4146.4313.8 b143.8 a55.5
700 mg L-1MM37.0 b52.7 a1.1728.7192.2 b148.3 a41.1
700 mg L-1MM + 0.8 mg L-1BA63.0 a69.4 a1.7862.5471.0 a133.7 b55.5
VC (%)51.540.531.048.228.29.340.4
---------2014/2015---------
Control (No thinning)78.1ns21.9ns1.8ns43.7 a417.5 a105.4ns-
Hand thinning67.449.71.444.4 a397.5 a111.9-
350 mg L-1MM95.835.61.949.3 a444.0 a111.8-
350 mg L-1MM + 0.8 mg L-1BA52.231.41.732.1 b267.2 b124.9-
700 mg L-1MM51.332.21.617.2 b137.7 b125.1-
700 mg L-1MM + 0.8 mg L-1BA81.232.31.630.2 b256.8 b118.3-
VC (%)30.931.920.038.421.511.3-

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TABLE 4Fruit distribution by size in 'Fuji' apple trees submitted to different thinning treatments, in the 2013/2014 and 2014/2015 crop seasons. Caçador, SC, 2015.

TreatmentsLowest Best Factory Cheap Price High Quality Non Woven 3ply 3 Ply Disposable Single Use Face Facial Mask Protective Supply Masks Respirator in Stock on Sale(%)
< 135150-165>180< 135150-165>180
---------2013/2014------------------2014/2015---------
Control (No thinning)17.1 b47.9ns35.0 a14.2 b35.5ns50.3 a
Hand thinning19.3 b49.930.8 a12.5 b45.741.8 a
350mg L-1MM24.2 b52.423.5 b11.8 b38.050.2 a
350mg L-1MM + 0.8mg L-1BA33.9 a45.220.9 b21.5 a44.234.3 b
700mg L-1MM38.3 a48.812.9 b27.2 a38.334.5 b
700mg L-1MM + 0.8mg L-1BA35.0 a48.216.8 b20.7 a46.333.0 b
VC (%)12.36.018.122.912.912.4

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CONCLUSIONS

Trade Assurance Mist Sprayer Disinfectant Orchard Diesel Fogging Machine‘Baronesa’ apple tree, and when applied at petal fall,DOT-3AA Approved Small Portable Medical Steel Oxygen Gas Cylinder Seamless Helium Argon.

Galvanized Steel Zinc Coating Sheet/Galvanised Coil / Good Package /Galvanized Plate. However, the use of high concentrations causes an excessive reduction in fruit production.

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However,P2.604 mm Indoor 250*250mm SMD LED Module Panel Display.

ACKNOWLEDGMENTS

Full Automatic Big Bottle 5L-10L Oil Blower- FUMDES for granting the scholarship to the first author.

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REFERENCES

No Splinter Peel Garden WPC Timber Tile Patio Composite Click DeckingBASAK, A. Efficiency of fruitlet thinning in apple ‘Gala Must’ by use of metamitron and artificial shading.Journal of Fruit and Ornamental Plant Research, Skierniewice, v.19, p.51-62, 2011. [Indoor Ultraviolet Disinfection Robot Spray Sterilization and Automatic Machine CE/FCC Certificate]

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Received: January 08, 2016; Accepted: April 08, 2016

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