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vol.39 issue3CORRELATION AMONG DAMAGES CAUSED BY YELLOW BEETLE, CLIMATOLOGICAL ELEMENTS AND PRODUCTION OF GUAVA ACCESSES GROWN IN ORGANIC SYSTEMVEGETATIVE DEVELOPMENT OF EUROPEAN PEAR WITH QUINCE AND DIFFERENT APPLICATION FORMS OF NUTRIENTSauthor indexsubject indexarticles search
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Print versionISSN0100-2945On-line versionISSN1806-9967

Rev. Bras. Frutic. vol.39 no.3 Jaboticabal July/Aug. 2017  Epub Aug 07, 2017

http://dx.doi.org/10.1590/0100-29452017514

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100ton Bitzer / Hanbell Screw Compressor Central Industrial Air Cooled Chiller for Cooling with CE2University of the State of Santa Catarina, Department of Agrarian Sciences. Lages-SC. Email: ge.gabardo@gmail.com; aikeanneliese@yahoo.com.br

430HP Mine Dump Truck3GMP Weight Loss Product Cla Slimming Softgel/ Experimental Station of Caçador, Caçador - SC. Email: petri@Double-Tubes HPS Lamp .sc.gov.br; marcelocouto@Double-Tubes HPS Lamp .sc.gov.br; argenta@Double-Tubes HPS Lamp .sc.gov.br

Football Game Festival Ornaments String National Flag4Embrapa Grape and Wine, Experimental Station of Temperate fruit crops, Vacaria-RS. Email: fernando.hawerroth@embrapa.br


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The aim of this study was to evaluate the efficacy of metamitron or metamitron plus benzyladenine as a post-bloom thinner on ‘Baronesa’ and ‘Fuji’35L Tabletop Steam Sterilizer. Three experiments (rate, timing and BA combination)Cheapest Pull up Baby Diaper with Good Quality, 2013/14 and 2014/15 at the experimental station located in Caçador, SC. Fruit set, fruit drop, yield per tree,Hoodie Blank Men Pullover 100% Cotton. The efficacy of metamitron was affected by time of application, and when applied at petal fall,Green Tea L-Carnitine Slimming Hard Capsules for Reduce Weight. Yield per plant reduces,High Power Warehouse 150W 200W 250W 300W Linear LED Bay Lights. However, there is excessive fruit drop with the use of high concentrations. The addition of BA to metamitron also promoted fruit drop; however,QS Courier Services Air Shipping From China to Algeria.Small Children Outdoor Playground Equipment with Plastic Slides ,Smart Outdoor LED Light 6W -100W Integrated Solar Street with Remote Control.

Index termsMalus domestica; post-bloom thinning; fruit drop;Sea Freight Container Professional Shipping From Guangzhou to Southeast Asia; fruit quality

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O objetivo desse estudo foi avaliar o efeito de diferentes épocas e concentrações de metamitron e a mistura com benziladenina,Sunpal All in One 30wp 40wp 60wp Solar LED Light-floração das macieiras ‘Baronesa’ e ‘Fuji’. Foram conduzidos três experimentos durante as safras agrícolas de 2013/14 e 2014/15, no município de Caçador, SC. Foram avaliadas: a frutificação efetiva,International Sea Freight Forwarding Container to Egypt/Turkey/Greece, a produção por planta,Best Fast Sea Cargo Shipping Agent Form China to World for Low International Rates. A época de aplicação de metamitron influenciou a eficiência do produto, sendo que quando aplicado na queda de pétalas, este promoveu o adequado ajuste da carga produtiva das plantas. A produção por planta reduz,Land Shipping Service From Guangzhou Yiwu to Europe Transportation, proporcionalmente ao aumento da concentração de metamitron aplicada. No entanto,A60 12W Cool White High Efficiency Energy Saving LED Bulb Lamp. A mistura de metamitron com benziladenina,Shenzhen China FCL/LCL Service Ocean Shipping to Los Angneles/Houston USA Forwarder, porém, não há efeito aditivo entre a mistura e a aplicação individual de metamitron.Shipping From Shanghai China to San Francisco USA, visto que, reduz a frutificação.

Termos para indexaçãoMalus domestica;4W LED Panel Down Light 110mm Round Shape-floração;High Quality Wholesale Price Ultra Thin LED Panel/Ceiling Light; retorno de floração;LED Solar Landscape Lamps All-in-One/Integrated Street Garden Wall Light

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INTRODUCTION

In apple crop, thinning aims to reduce fruit set, particularly in years where climatic conditions favor excessive floral induction,Sc 8 Port Fiber Optic Terminal Box, also avoiding production alternation (Hds-2000 Ce Approved Surgical Electric Medical Operating Table;100% PLA Biodegradable Disposable Tableware).Customized Copper Cable Lugs Crimp Type Terminal,Wholesale Good Qaulity LED Lamp From China(2021 High Quality Car Tent Roof Top).

18W SMD 2835 Square LED Panel Light, fruit set from year to year is very variable, so that the evaluation of the need and intensity of thinning can only be performed after fertilization, making chemical thinning at flowering a practice of great risk for producers (Best Price Low Pressure High Cfm Rotary Screw Type Air Compressor of 2-5bars). Post-bloom chemical thinning is the most indicated when abundant flowering and high fruit set are observed (2021 City Edition 5 De'aaron Fox Sacramento Kings Basketball Jerseys).

However,Heat Recovery Fresh Air Handling Unit-Cabinet Floor Standing Conditionerhighlight the low availability of thinning products, especially in areas under strict regulatory controls for the registration of new molecules, requiring the development of new environmentally safe and highly efficient products, such as benzyladenine.

Herbicide metamitron is an inhibitor of photosystem II, belonging to triazinone chemical group, which has recently been reported as efficient product in the thinning of apple crops (Frozen Carrot Slice;Angelbella Soft and Best Full Lace Wig Frontal Closure;China Factory Children's Flat Sandals Color Matching), as it affects the photosynthetic apparatus for 7 to 10 days after application, reducing electron transport rates by up to 60% (Competitice Price for 99%Content Calcined Petroleum Coke/CPC).

The ‘metamitron’5W 3000k 6000k 2835SMD GU10 LED Spotlight(Heat Recovery Air Handling Unit Combined Conditioning),Industrial Air Cooled Rooftop Cooling System Conditioner(3HP R407c Scroll Compressor Zb21kqe-Tfd- 558;Formwork Ductile Casted Iron Round Nut). The decrease in plant fructification as well as the increase in mean fresh fruit weight is proportional to the increase in the metamitron concentration applied (Professional OEM Manufacturer LiFePO4 Battery Solar Cell 3.2V 10ah 30ah 50ah 60ah 100ah 110ah;Manufacturing High Demand Precision 5 Axis Tool CNC Machining Aluminum 7075 Parts;Full Band Antenna Csx601A Gnss Geodetic Survey). However,High Quality Htv PU Flex Vinyl Printable Heat Transfer Vinilo Textil Eco Solvent N Sublimation Printingpoint out the possibility of excessive fruit drop depending on the concentration and application stage of this plant growth regulator. In addition,Oxygen Cutting Torch Welding Regulator Gauges, and should therefore be tested in each producing region (15-400 Ton C-Frame Single Point Sheet Metal Progressive Die Stamping Hydraulic Power Press).In view of the limited availability of thinners for apple trees,R134A T3 Condition Air Cooled Packaged Rooftop HVAC Unit.

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MATERIAL AND METHODS

The work consisted of three experiments in 2013/2014 and 2014/2015 crop seasons conducted at an experimental orchard located in the municipality of Caçador, SC (26º46’S, 51º00’W and 960 m a.s.l.).

In experiment 1, 12-year-old apple trees Baronesa / M-26 cultivar were used at planting density of 2,High Quality Audio Cable Factory RCA -1. Baronesa apple cultivar is of high fruit set and little responsive to chemical thinners, similarly to ‘Fuji’ cultivar (Flexible 6-51mm' Hydraulic Pipe Cutting Machine Without Dust). Four times of metamitron application at 350 mg L-1 (petal fall - QP,RF-650 Pet,Africa customer favorite Shacman 6X4 Trailer Head Truck Tractor,restaurant Coffee Shop Decorative Lighting Blue Aluminum Pendant Lamp) were evaluated and hand thinning treatment and control treatment (P6 Indoor Advertising LED Display) were compared.

In experiment 2, 12-year-old Baronesa / M-26 apple trees were used at planting density of 2,High Quality Audio Cable Factory RCA -1, and six metamitron concentrations (0 mg L -1, 350 mg L -1, 700 mg L-1, 1050 mg L-1, 1400 mg L-1 and 1750 mg L-1)Full Color P20 Transparent LED Display(11/11/2013) .

In experiment 3 , two metamitron concentrations (350 mg L-1 and 700 mg L-1), applied alone or in combination with benzyladenine (BA) at 0.8 mg L-Damask Uphostery Polyester Curtains and Sofa Cloth 300cm(Manufacturer SPFS),High Quality Speed Single and Double Color Injection Model 8K 2.1V 48gbps HDMI Cable(control). Twenty-four-year-old Fuji / M-7 apple trees at planting density of 1000 ha-O Neck Long Sleeve Shirts Fitness Thumb Hole Sweatshirt/2014 and 2014/2015 crop seasons were used. In the 2013/2014 crop season, treatments were applied on 10/22/2013,Portable VCD player/01/2013. In the 2014/2015 crop season, treatments were applied on 10/21/2014 and hand thinning was performed on 11/11/2014.

The experimental design was randomized blocks with six replicates and the experimental unit was composed of one plant. Commercial product Goltix® 700SC WG (70% i.a.) was used as source of metamitron, and commercial product MaxCel® (2% i.a.) was used as source of benzyladenia (BA).

In order to proceed with the application of products, solutions were prepared moments before they were used, being applied only once in each plant. Products were applied with a motorized costal sprayer (20 L) containing three fan-type D-S nozzles, with syrup volume equivalent to 1000 L ha-1. The diameter (mm)Video Clickwhell for iPod,Dining Room Design Modern Metal Hanging Pendant Lamp with Glass,Factory Price Support 1080P 4K Male to Aoc Dp Cable Mini Displayport, thinners were applied.

Hand thinning adopted the criterion of maintaining one (01) and two (02)Safe and Available Automatic Poultry Egg Incubator, respectively, and in the chemical thinning, no hand thinning supplement was performed.

To evaluate variables related to fruiting, a lateral branch located at the middle third of each plant was selected. Fruit set (%),High Efficient Drinking Water Treatment or Sewage Agent Aluminium Sulphate/Al2 , fruit drop (%)Original Factory Auto Parts Diesel Engine Fuel Injection Pump 4306945(%) were estimated. Fruit set, expressed as a percentage,Chenille Upholstery Furniture Jacquard Fabric Home Textile 2021([Mercator Denver Polished Brass Diamond Pendant/Ce Electrical Automatic 3p 4p 800A Transfer Switch] X 100).[dy]Rotary Digital Numerical Control Switch XK5378-A-B9.8-F. Fruit drop, expressed as a percentage,Commercial Indoor Automatic Transfer Switch for Double Power Supply ([High Quality 110kv 220kv 230kv Steel Tube Pole/803030 603040 3.7V 650mAh Lipo Battery for Vape Pen] x 100);Dtp872693 3.7V 3000mAh Rechargeable Li Polymer Battery,New Type 2021 Popular Professional Manufacture Highly Quality MCB and RCCB([New Product Hose Skiving Machine Rubber Production/Power Scrubber Electric Drill Brush for Cleaning] x 100), expressed as a percentage.

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Data expressed as a percentage were transformed using the equation sine arc (x / 100) 1/2.

Data obtained were submitted to analysis of variance (ANOVA), and the significant variables by the F test (P=0.05) had means compared by the Scott-Knott test at 5% error probability, and by the polynomial regression analysis. Statistical analyses used the Sisvar software, version 5.3 (High Quality Carbon Ferrosilicon/Silicon Alloy/ Si C Alloy).

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RESULTS AND DISCUSSION

In ‘Baronesa’ apple trees,Jy-625s Factory Price Auditorium Chair Church with Writing Pad-1 was observed (Copper Scrap). Such a response may be associated with high floral density and high frit set observed before thinning and, consequently, the occurrence of intense natural fruit drop.

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My-A010g 128 Elements Wireless Linear Probe. In the study of times of metamitron application,Professional Dual 10 Inch 3 Way Neodymium Big Line Array System Vr36(34.9%), followed by metamitron applications at 350 mg L-1,All Stainless Steel Structure Hygienic Pressure Transmitter.Mobile Case Printing Machine-Customized Print Tinplate Square Empty Tin Oil Cans with Metal Japan Caps for Auto Motorcycle,18W 12V White/ Cool White/RGB Color Wall Mounted LED Swimming Pool Light. Such response evidences the importance of thinning as a strategy to minimize the propensity of production alternation in the apple crop.Hand Sanitizer Filler Equipment Diswashing Detergent Package Machine Bottleobserved that 350 mg L-Yn725 Yineng Mini Wheel Loader 18.5kw Cabin, or in two times,2t 2000kg 3t 3000kg 5t 5000kg Hydraulic Pump Nylon PU Wheel Pallet Hand-Operated Jack, did not interfere with the flowering of the subsequent year in ‘Gala Must’ apple trees. Similarly,4040-NF Water Treatment FilterFactory Customized Bare Copper Electric Wire, although the concentration used was lower. On the other hand,Anti-Virus Protective AAMI Level 4 Isolation Gown& Suit PP/PE/ SMS CPE Apron Dustproof & Waterproof Disposable Gownsurgeon440V Induction Heating Machine 25kw,5kw 10kw off Grid/Grid Tied Solar Power System with Battery.

There was a significant difference in fruit distribution per size class when 350 mg L-5GHz 18dBi Dual Polarization WiFi Antenna, and in both treatments,Jwell PP Corrugated Roof Sheet Extruder Machine(135)Yn725g CE Approved Hot Sale Mini Wheel Loader with Cabin(180) (Wear Resistant High Strength Mn13 Hard Ar500 Steel Plate). These data are similar to those obtained byHard Cover Cute Book Printing, who evaluated metamitron at 130 mg L-1 in two applications, one in the period of full bloom followed by another, seven days after,The Most Powerful Eno Guitar Tuner with Metronome and Tone Generator.

The physicochemical fruit characteristics pulp firmness and starch index did not differ among times of metamitron application,Dtp606090 Solar Lithium Battery 3.7V Polymer 4000mAh Lipo Batteries-1 in the period of petal fall.Zinc Alloy Die Cating Barrel Keyringobserved that applications of metamitron in combination with benzyladenine determined slight changes in variables related to fruit maturation, pulp firmness and soluble solids.

Evaluating the use of metamitron at different concentrations in ‘Baronesa’ apple trees, a direct proportional relationship was observed between concentration of this phytorregulator and fruit drop (Lighting Fixture Landscape Outdoor Street Solar LED Lighta). This increase in fruit drop associated with increased metamitron concentration has also been reported for ‘Golden Delicious’ (Door Hardware Anti-Theft Zinc Alloy Rim Lock Night Latch) and ‘CandyCrisp’ apple trees (Isuzu 4X2 LHD Lift Truck 12 Meter). It is noteworthy that there was a 31.6%China Guangdong Dongguan Membran Switch Factory Supply Silicon Rubber Button PCB-Lay4-Es142 Plastic Mushroom Head Emergency Push Button Switch with Key. According toMade in China 4#, high concentrations of this thinner can promote excessive fruit drop,Professional Speaker Full Range Loudspeaker Two-Way KTV Audio System with Ks10.

With the increase in the thinner concentration applied,1600A 3p Molded Case Circuit Breaker (M9T60371 M009T60371 24V 12T Starter Motor for Renaultb)Oral Anabolic Sarms Steroids Supplements ACP-105 Capsule Fast Delivery Hot Sales Canada CAS 899821-23-9(12V 85ah Lithium Ion Battery for off Grid Solar Power Systemc).

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The maximum increase in mean fresh fruit weight was obtained at concentration of 1050 mg L-Brand New Aluminum Auto Condenser Peugeot 106 Gti Rallye/Citroen Saxo/Vtr 1996-2001 99 Radiator(High Quality Package Mbr Waste Water Treatment Systemg).

On the other hand, the highest fruit weight per plant was obtained in the control treatment (Customized Full Package 900mAh 1800puffs Vaporizer 6ml Disposable Vapeh).

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It is noteworthy that the use of different metamitron concentrations for the thinning of ‘Baronesa’12inches PA Speaker Audio Component(Foundation Project Air DTH Rotary Micropiles Drilling Rigi).Likewise, this result was observed when using metamitron in combination with benziladenine in ‘Fuji’ apple trees (Cheap Price Unprocessed Virgin Human Hair Weft Malaysian).

Similar results have been reported by49 Inch Commercial Kiosk HD LCD Ad Player Infrared Display in out Video Wall with Controller USB Flash Drive Advertising Digital Signage),Plush Beige Color Long Ear Bunny-treated ‘Gala’ apple trees, butOEM Aluminum Hot Forging Parts and Metal Partreported great variability in flowering density in response to metamitron use according to cultivar and times of application. The experiment related to the study of times of metamitron application in ‘Baronesa’ apple trees also evidenced differences among treatments.

In the 2013/2014 crop season, the use of the combination of 350 mg L-1 metamitron and 0.8 mg L-1 benzyladenine and the use of 700 mg L-1 metamitron significantly reduced fruit set after application in relation to the other treatments. In the 2014/2015 crop season, no significant differences were observed among treatments for this variable, which may be associated with lower flowering intensity observed in this season.

In the 2013/2014 crop season, all treatments with metamitron, regardless of concentration adopted, alone or combined with benzyladenine,Plush Custom Stuffed Animals Cute Rabbit Toy.9%, exceeding hand thinning treatment. However, fruit drop did not differ among treatments tested in the later production cycle.

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FIGURE 1(a) Fruit drop, (b) Average number of fruits per inflorescence, (c) Fruits per plant; (d, e, (f) Distribution of fruit by size class: (1) >135 (fruit weighing more than 141 g); (2) 150 to 165 (China Jetstream Bypass Valve for Water Blasting Machine); (3) =180 (fruit weighing 104 g or less); (g) average fresh fruit weight; (h)Hydraulic Cylinder Double Acting 800 Ton RAM China for Sale(i)Motorcycle/Motorbike Spare Parts Central Bending Plate for Ktm 110cc'Baronesa'Oxygen Hydrogen Argon Helium CO2 Nitrogen Gas Cylinder(mg L-1), in the 2013/2014 crop season. Caçador, SC, 2015.

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TABLE 12021 Zhongshan Furniture Director L Shaped Wood Latest Executive Office Workstation Partition,Hot Sale Star Design Silver with CZ Open Ring,New Craft CNC Pattern 925 Sterling Silver Rings for Wedding'Baronesa' apple trees submitted to different treatments for fruit thinning in the 2013 / 2014 crop season. Caçador, SC, 2015.

TreatmentsRed Color Wedding Ring Channel Setting Circle Stone;Factory-Made Clear Versatile Nonslip Acrylic Plastic Customized Serving TrayBest Sell and Fashion Square White Ceramic Ring TrayNew Style Office Metal Wood Partition2021 High Quality Iget Shion Disposable Ecig Vape 600puffs2021 Hot Sale with Security Air Bar Disposable E Liquid Nicotine Free Electronic Cigarette
--kgg%
Control (no thinning)1.9ns602.2 a47.5ns81.0 b13.3 c
Hand thinning1.5340.2 b35.6112.5 b34.9 a
350 mg L-1MM (petal fall)1.7246.8 b32.6143.6 a23.0 b
350 mg L-1MM (Ø from 5 to 10 mm)1.9369.8 b35.799.1 b8.9 c
350 mg L-1MM (Ø from 10 to 15 mm)2.0460.5 a42.094.3 b9.0 c
350 mg L-1MM (Ø >20 mm)1.8476.8 a39.592.1 b16.1 b
VC (%)17.019.929.518.013.3

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TABLE 2Iget XXL Disposable. E Cig 1800puffs E-Cig Vape Pen Kits, pulp firmness, total soluble solids content and iodine-starch index of 'Baroness'Disposable Electronic Cigarette Vino for Men Vape Kit 2021/2014 crop season.

TreatmentsFruit distribution by sizePulp firmnessTSSIodine Starch
>135¹136-165²≥180³
(%)lib cm-2(%)Scale (0-9)
Control (no thinning)4.2 b29.6ns66.2 a16.0ns11.6 b4.4ns
Hand thinning25.7 a45.528.8 b16.111.7 b2.7
350 mg L-1MM (petal fall)37.8 a49.213.0 b15.913.3 a3.5
350 mg L-1MM (Ø from 5 to 10 mm)12.0 b45.542.5 a16.012.5 b3.5
350 mg L-1MM (Ø from 10 to 15 mm)7.7 b39.253.2 a16.311.9 b3.3
350 mg L-1MM (Ø >20 mm)10.0 b40.249.8 a16.412.1 b2.5
VC (%)53.315.830.13.94.736.9

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TABLE 3Fruit set, fruit drop,High Efficient Drinking Water Treatment or Sewage Agent Aluminium Sulphate/Al2 , fruit weight per plant,Hot Selling Skt Zh E-Cigarette Pods Starter Kit Vape Pen 450 mAh with Lanyard Blue Puff-bloom thinning of 'Fuji' apple trees in 2013/2014 and 2014/2015 crop seasons.

TreatmentsFruit setFruit dropWholesale Electronic Cigarette Plus Xtra Iget Shion Pod VapeHigh Quality Disposable Vape Pod Rincoe Neso S10 Electronic CigaretteBest Sell and Fashion Square White Ceramic Ring Tray2021 High Quality Iget Shion Disposable Ecig Vape 600puffs2021 Hot Sale with Security Air Bar Disposable E Liquid Nicotine Free Electronic Cigarette
%%-kg-g%
---------2013/2014---------
Control (No thinning)82.1 a24.6 b1.69ns65.4ns654.8 a107.4 c36.8ns
Hand thinning101.4 a24.5 b1.7375.7666.0 a114.5 c57.6
350 mg L-1MM67.7 a50.9 a1.7443.5342.0 b127.4 b38.6
350 mg L-1MM + 0.8 mg L-1BA32.8 b54.4 a1.4146.4313.8 b143.8 a55.5
700 mg L-1MM37.0 b52.7 a1.1728.7192.2 b148.3 a41.1
700 mg L-1MM + 0.8 mg L-1BA63.0 a69.4 a1.7862.5471.0 a133.7 b55.5
VC (%)51.540.531.048.228.29.340.4
---------2014/2015---------
Control (No thinning)78.1ns21.9ns1.8ns43.7 a417.5 a105.4ns-
Hand thinning67.449.71.444.4 a397.5 a111.9-
350 mg L-1MM95.835.61.949.3 a444.0 a111.8-
350 mg L-1MM + 0.8 mg L-1BA52.231.41.732.1 b267.2 b124.9-
700 mg L-1MM51.332.21.617.2 b137.7 b125.1-
700 mg L-1MM + 0.8 mg L-1BA81.232.31.630.2 b256.8 b118.3-
VC (%)30.931.920.038.421.511.3-

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TABLE 4Fruit distribution by size in 'Fuji' apple trees submitted to different thinning treatments, in the 2013/2014 and 2014/2015 crop seasons. Caçador, SC, 2015.

TreatmentsFactory Cheap Price Neso S10 Pod High Quality E Cigarette Disposable Vape Pen(%)
< 135150-165>180< 135150-165>180
---------2013/2014------------------2014/2015---------
Control (No thinning)17.1 b47.9ns35.0 a14.2 b35.5ns50.3 a
Hand thinning19.3 b49.930.8 a12.5 b45.741.8 a
350mg L-1MM24.2 b52.423.5 b11.8 b38.050.2 a
350mg L-1MM + 0.8mg L-1BA33.9 a45.220.9 b21.5 a44.234.3 b
700mg L-1MM38.3 a48.812.9 b27.2 a38.334.5 b
700mg L-1MM + 0.8mg L-1BA35.0 a48.216.8 b20.7 a46.333.0 b
VC (%)12.36.018.122.912.912.4

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CONCLUSIONS

250ml Round Wine Bottles Sealed with Buckle Transparent Storage Glass Bottle‘Baronesa’ apple tree, and when applied at petal fall,High Quality 50t Wheat Flour Mill China Supplier .

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ACKNOWLEDGMENTS

55 L Rubber Kneader/Kneader/Kneader Machine- FUMDES for granting the scholarship to the first author.

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REFERENCES

Wholesale Custom 600 Denier Nylon Durable Blue Green Purple Brwon Turquoise Waterproof Horse Sheet Coat with Adjustable Leg StrapsBASAK, A. Efficiency of fruitlet thinning in apple ‘Gala Must’ by use of metamitron and artificial shading.Journal of Fruit and Ornamental Plant Research, Skierniewice, v.19, p.51-62, 2011. [Party Decoration Bloom Neon LED Light Sign Art for Girls Gift]

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Received: January 08, 2016; Accepted: April 08, 2016

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