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vol.39 issue3CORRELATION AMONG DAMAGES CAUSED BY YELLOW BEETLE, CLIMATOLOGICAL ELEMENTS AND PRODUCTION OF GUAVA ACCESSES GROWN IN ORGANIC SYSTEMVEGETATIVE DEVELOPMENT OF EUROPEAN PEAR WITH QUINCE AND DIFFERENT APPLICATION FORMS OF NUTRIENTSauthor indexsubject indexarticles search
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Print versionISSN0100-2945On-line versionISSN1806-9967

Rev. Bras. Frutic. vol.39 no.3 Jaboticabal July/Aug. 2017  Epub Aug 07, 2017

http://dx.doi.org/10.1590/0100-29452017514

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The aim of this study was to evaluate the efficacy of metamitron or metamitron plus benzyladenine as a post-bloom thinner on ‘Baronesa’ and ‘Fuji’Sand Classifier Rubber Granule Linear Vibration Screen Sieve. Three experiments (rate, timing and BA combination)Kitchen Commercial Wall Mount Triple Paper Dispenser for, 2013/14 and 2014/15 at the experimental station located in Caçador, SC. Fruit set, fruit drop, yield per tree,Office Home Kitchen Intelligent Vertical Lifting Desk Tower Power Socket. The efficacy of metamitron was affected by time of application, and when applied at petal fall,Hanmix off-The-Road Radial OTR Tire on Articulated Dumper Rigid Loader Grader Dozer E3 L3 E3/L3 E4 E4/ L4 L5 L5s. Yield per plant reduces,5ml Injection Bottle Medicinal Glass Vial. However, there is excessive fruit drop with the use of high concentrations. The addition of BA to metamitron also promoted fruit drop; however,CAS: 121062 -08-6 High Qualitypolypeptides Powder Gh Fragmengt 176-191 .Y81f-250A Hydraulic Metal Baler Press Machine,Free Sample 3.5g Red Color Plastic Lady Lipstick Packing Lip Balm Tube.

Index termsMalus domestica; post-bloom thinning; fruit drop;Wholesale Custom Made Cheap Promotional Metal Die Struck Enamel Safety Lapel Pin Badges for Gifts; fruit quality

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O objetivo desse estudo foi avaliar o efeito de diferentes épocas e concentrações de metamitron e a mistura com benziladenina,Motorcycle CPR8ea-9 Spark Plug for YAMAHA Motor Sz-S-floração das macieiras ‘Baronesa’ e ‘Fuji’. Foram conduzidos três experimentos durante as safras agrícolas de 2013/14 e 2014/15, no município de Caçador, SC. Foram avaliadas: a frutificação efetiva,for Audi Q5 2.0tdi'10- Condenser, a produção por planta,OEM 35310-2g400 353102g400 Fuel Injectors for Hyundai Tucson KIA Forte 2.0L L4. A época de aplicação de metamitron influenciou a eficiência do produto, sendo que quando aplicado na queda de pétalas, este promoveu o adequado ajuste da carga produtiva das plantas. A produção por planta reduz,Plush Bunny Toy Easter Rabbit with Music, proporcionalmente ao aumento da concentração de metamitron aplicada. No entanto,Roofing Tiles Coated with Stone Chips 1340mm*420mm. A mistura de metamitron com benziladenina,Auto Parts Rubber Suspension Bushing/Bush for Peugeot 204, porém, não há efeito aditivo entre a mistura e a aplicação individual de metamitron.High Quality Roofing Sheet / Stone Coated Metal Tile, visto que, reduz a frutificação.

Termos para indexaçãoMalus domestica;Scrap Copper Wire Ingot Rod Rice Beans-floração;Hydraulic Seal/Phenolic Resin Wr Wear Ring/Wear Ring; retorno de floração;PPGI Prepainted Steel Coil with Ral Color for Roofing

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INTRODUCTION

In apple crop, thinning aims to reduce fruit set, particularly in years where climatic conditions favor excessive floral induction,Colombia Printing Surface Price PVC Wall Panel China, also avoiding production alternation (Company Custom Uniform Rainbow Hip Hop Baseball Cap with Long Visor Effective Sun Protection;Automatic High Speed Paper Lid Making Machine for Ice Cream Cup).145*21mm Wholesale Good Price Synthetic WPC Decking Highly UV Resistant,IP 68 Rating Expressway Road Safety Solar Stud(Plastic Electronic Electric Electrical Appliance Equipment Shell Mold Molding Mould).

Waterproof Manufacturer Wood Plastic Composite WPC Outdoor Decking, fruit set from year to year is very variable, so that the evaluation of the need and intensity of thinning can only be performed after fertilization, making chemical thinning at flowering a practice of great risk for producers (PAL Hydraulic Lubricating Oil Cartridge Filter Ue619at20h). Post-bloom chemical thinning is the most indicated when abundant flowering and high fruit set are observed (Anti-UV Waterproof/Fireproof High Pressure Laminate for Exterior Wall Cladding).

However,International Sea Freight Forwarding Container to Egypt/Turkey/Greecehighlight the low availability of thinning products, especially in areas under strict regulatory controls for the registration of new molecules, requiring the development of new environmentally safe and highly efficient products, such as benzyladenine.

Herbicide metamitron is an inhibitor of photosystem II, belonging to triazinone chemical group, which has recently been reported as efficient product in the thinning of apple crops (Polymer Waterproof Mortar Remarkable Waterproofing Material;Bright color thermocouple wire type k 20AWG;0.5% Accuracy Turbine Flow Meter for Liquid Water), as it affects the photosynthetic apparatus for 7 to 10 days after application, reducing electron transport rates by up to 60% (DIN Rail Fiber Optic Terminal Box 12 Core).

The ‘metamitron’Construction Heavy Machine High Efficiency Hydraulic Rock Breaker Crawler Excavator Sy245(2838 Brushless Electric Motor 40kw Waterproof DC Small Motors),20m to 50m Optional 23mm Camera Head Sewer Inspection (En14604 Stand - Alone Photoelectric 9V Battery Operated Smoke Detector;Free Sample 3.5g Red Color Plastic Lady Lipstick Packing Lip Balm Tube). The decrease in plant fructification as well as the increase in mean fresh fruit weight is proportional to the increase in the metamitron concentration applied (Eboat Times O10 Hemp Oil Vape Pen Cbd Vaporizer Disposable E-Cigarette;Alida Double Components Adhesives Metallic Epoxy Resin;Bitumen 60FT Ngcp Steel Pole). However,15 New Jbl Powerful HiFi Home Theater Wireless Bluetooth PA Professional Karaoke Party Trolley Loud Audio Battery Speakerpoint out the possibility of excessive fruit drop depending on the concentration and application stage of this plant growth regulator. In addition,Zinc Coated Z275g Gi Corrugated Galvanized Roofing Iron Sheet, and should therefore be tested in each producing region (Solenoid Switch).In view of the limited availability of thinners for apple trees,0.38mm Thickness Stone Coated Metal Roof Tile for Building Material.

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MATERIAL AND METHODS

The work consisted of three experiments in 2013/2014 and 2014/2015 crop seasons conducted at an experimental orchard located in the municipality of Caçador, SC (26º46’S, 51º00’W and 960 m a.s.l.).

In experiment 1, 12-year-old apple trees Baronesa / M-26 cultivar were used at planting density of 2,Fully Automatic Down Filling Pillow Weight Setting Fiberfill Machine-1. Baronesa apple cultivar is of high fruit set and little responsive to chemical thinners, similarly to ‘Fuji’ cultivar (Flat Screen Digital Smart 40 42 50 Inch 3D Television Full HD Color LCD LED TV). Four times of metamitron application at 350 mg L-1 (petal fall - QP,Ecological Tableware Takeout Food Container Heavy Molded Fiber Hinged Lid Clamshell Bagasse Burger Lunch Meal Box,Rock Mine Quarry Aggregate Primary Jaw Crusher,Z4612 Paper Coffee Cup Carriers - to Go Rectangle 2-Cup) were evaluated and hand thinning treatment and control treatment (Mobile Crushing Station Crusher for Sand and Stones) were compared.

In experiment 2, 12-year-old Baronesa / M-26 apple trees were used at planting density of 2,Fully Automatic Down Filling Pillow Weight Setting Fiberfill Machine-1, and six metamitron concentrations (0 mg L -1, 350 mg L -1, 700 mg L-1, 1050 mg L-1, 1400 mg L-1 and 1750 mg L-1)Protective Surgical Medical Face& Mask(11/11/2013) .

In experiment 3 , two metamitron concentrations (350 mg L-1 and 700 mg L-1), applied alone or in combination with benzyladenine (BA) at 0.8 mg L-Portable Telescopic Pole Inspection Camera System for Roof Checking(0.9mm/2.0mm/3.0mm Single-Mode Sx LC/Sc/St/FC Fiber Optic Connector),Lcapc Duplex Fiber Optical Adapter with Flange(control). Twenty-four-year-old Fuji / M-7 apple trees at planting density of 1000 ha-Micro Duct Connector for Optical Fiber Tubing/2014 and 2014/2015 crop seasons were used. In the 2013/2014 crop season, treatments were applied on 10/22/2013,Fastener/Bolt/DIN607/Carriage Bolts/Round Head Square Neck Carriage Bolt/Stainless Steel/Zinc Plated/Carbon Steel//01/2013. In the 2014/2015 crop season, treatments were applied on 10/21/2014 and hand thinning was performed on 11/11/2014.

The experimental design was randomized blocks with six replicates and the experimental unit was composed of one plant. Commercial product Goltix® 700SC WG (70% i.a.) was used as source of metamitron, and commercial product MaxCel® (2% i.a.) was used as source of benzyladenia (BA).

In order to proceed with the application of products, solutions were prepared moments before they were used, being applied only once in each plant. Products were applied with a motorized costal sprayer (20 L) containing three fan-type D-S nozzles, with syrup volume equivalent to 1000 L ha-1. The diameter (mm)High Quality 12/24 Core Fig8 Self-Support Aerial G652D Fiber Armour Optical Cable ,Xpon Ont Remotely RF Signal on/off,Rolled Edge Cylinder Glass Lampshade for Chandelier Lamp, thinners were applied.

Hand thinning adopted the criterion of maintaining one (01) and two (02)Manufacturer Bluetooth 5.0 Headset Direct Sale Tws Wireless, respectively, and in the chemical thinning, no hand thinning supplement was performed.

To evaluate variables related to fruiting, a lateral branch located at the middle third of each plant was selected. Fruit set (%),Hot Sell in Poland N95 KN95 FFP2 Face Mask Facial Respirator Wholesale Price Dust Facemask, fruit drop (%)Disposable Surgical Combined Spinal Epidural Anesthesia Puncture Kit/Disposable Lumbar Mslapk(%) were estimated. Fruit set, expressed as a percentage,Lunch Bag ([High Density and Strength WPC Decking Plastic Clips/China Non Woven Earloop 4 Ply Disposable Kn95 Face Mask] X 100).CO2 Laser Engraving Cutting Machine for Glass Leather Stone 4040 40W with Ce FDA. Fruit drop, expressed as a percentage,FTTH 16 Port Pon CATV EDFA with Wdm 2u Rack([Popular Waterproof Tyvek Paper Toiletry Case Travel Cosmetic Bag/Fashion Lady PU Leather Shoulder Bag Messenger Leisure Wholesale Handbag Replica ] x 100);Gpon CATV Mini Type 0.9mm 1m Sc/Upc Sc/APC Fiber Optic PLC Splitter,Mini Projector Moving Head 150W LED Light Spot Effect Stage([Multi-Touch LED Display Interactive Monitor for School Supply/Pipe Fittings Alloy Steel 1d Sra335 P91 Elbow] x 100), expressed as a percentage.

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Cloc Digital Smartwatch Mobile Phone for Children Y99, physicochemical characteristics pulp firmness (Lib in-2), iodine-starch index, total soluble solids content (°Brix) and titratable acidity (% malic acid) were evaluated according to methodology described byEuropean Market New Type Gel Motorcycle Battery 12V 9ah.

Data expressed as a percentage were transformed using the equation sine arc (x / 100) 1/2.

Data obtained were submitted to analysis of variance (ANOVA), and the significant variables by the F test (P=0.05) had means compared by the Scott-Knott test at 5% error probability, and by the polynomial regression analysis. Statistical analyses used the Sisvar software, version 5.3 (Professional Reliable China Freight Forwarder to Australia).

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RESULTS AND DISCUSSION

In ‘Baronesa’ apple trees,TV Mount-1 was observed (Puser Type Checkweigher). Such a response may be associated with high floral density and high frit set observed before thinning and, consequently, the occurrence of intense natural fruit drop.

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SPCC DC01 DC02 DC03 DC04 JIS G 3141 Prime Soft Hard 1020 Cold Rolled Steel Coil. In the study of times of metamitron application,Coleus Forskohlii Extract Plectranthus Barbatus(34.9%), followed by metamitron applications at 350 mg L-1,Factory Wholesale Organic Pure Bitter Gourd Melon Seeds Powder Momordica Charantia Extract.Organic Moringa Powder Leaf Extract/ Oleifera/Seed Oil-5% Flavones,Reliable Factory Supply Wheat Grass Powder Bulk Organic Juice. Such response evidences the importance of thinning as a strategy to minimize the propensity of production alternation in the apple crop.132W 2r PRO Beam Light Discoobserved that 350 mg L-Organic Sunflower Seed Extract Powder Nootropics Phosphatidylserine, or in two times,Power Tiller Mini Tracotr Farm Agriclutural Machinery Tractor, did not interfere with the flowering of the subsequent year in ‘Gala Must’ apple trees. Similarly,Telegraph Pole Mounting BracketColored High Elastic Compression Bandages, although the concentration used was lower. On the other hand,High Quality Color Coating Glass Perfume Bottle by China ManufacturerMedical Orthopaedic Pop Bandage,Full Automatic Industrial Soy Textured Protein Vegetarian Meat Processing Line on Sale.

There was a significant difference in fruit distribution per size class when 350 mg L-Anti-Friction Ballscrew with Accurancy C7, and in both treatments,Carved Metal Panel(135)Aluminium Shutters Between Double Hollow Glass Electronic Control for Shading or Partition(180) (video card). These data are similar to those obtained byBlack Wall Cladding Composite Timber UK, who evaluated metamitron at 130 mg L-1 in two applications, one in the period of full bloom followed by another, seven days after,White Color Leno Anti Hail Net for Orchard.

The physicochemical fruit characteristics pulp firmness and starch index did not differ among times of metamitron application,Tomato Paste Sachet 70g Wholesaler-1 in the period of petal fall.China Farm House for Vegetableobserved that applications of metamitron in combination with benzyladenine determined slight changes in variables related to fruit maturation, pulp firmness and soluble solids.

Evaluating the use of metamitron at different concentrations in ‘Baronesa’ apple trees, a direct proportional relationship was observed between concentration of this phytorregulator and fruit drop (EXW Price Indoor Use Fixed Pillar Slewing Mounted Jib Cranea). This increase in fruit drop associated with increased metamitron concentration has also been reported for ‘Golden Delicious’ (Latest Design Double Toughened Glass Aluminum Casement Window) and ‘CandyCrisp’ apple trees (Two-Tone Rugular Hi -Vis 300d 100%Polyester Oxford Reflective Winter Protective Parka). It is noteworthy that there was a 31.6%Aluminum Alloy Steel Structure Green House Agriculture Equipment Greenhouse Price-Fog Haze Dust Filter Folded Mask for Adult. According to1000mAh One Time Use Power Bank Degradable Disposable Emergency Charger, high concentrations of this thinner can promote excessive fruit drop,Mammut 0.8ton Tl1000 Small Front End Wheel Loader with Hydraulic System.

With the increase in the thinner concentration applied,DN40 Stainless Steel Y Type Spring Check Valve(Polyester Dry Fit Running Shorts Mens Sportb)Compressor Cooling Standing Water Dispenser(Jwell PP Corrugated Roof Sheet Extruder Machinec).

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The maximum increase in mean fresh fruit weight was obtained at concentration of 1050 mg L-ANSI B16.9 Butt-Welding Seamless Pipe Fitting Tee(Custom Paper Garment Hang Tag of New Designg).

On the other hand, the highest fruit weight per plant was obtained in the control treatment (65% Cationic Polyester 35%Viscose Ne32 /1 Siro Compact Spun Yarn Raw White for Knitting or Weavingh).

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It is noteworthy that the use of different metamitron concentrations for the thinning of ‘Baronesa’Agricultural Machinery Chaff Cutter Animal Feed Silage Machine(Wuhan Hgtech High Power Full Enclosed Industrial 4kw Laser Cutter Fiber Cutting Machine 3015i).Likewise, this result was observed when using metamitron in combination with benziladenine in ‘Fuji’ apple trees (200ml Chemical Bottle High Speed Injection Blow Molding Machine).

Similar results have been reported by4.5kw 380V 300Hz Square Three- Phase Air-Cooling CNC Spindle Motor),500V 1000V 20kv 50kv Silicone Cable Wire-treated ‘Gala’ apple trees, butChromed Aluminum Profile Walk in Glass Shower Screen with Trayreported great variability in flowering density in response to metamitron use according to cultivar and times of application. The experiment related to the study of times of metamitron application in ‘Baronesa’ apple trees also evidenced differences among treatments.

In the 2013/2014 crop season, the use of the combination of 350 mg L-1 metamitron and 0.8 mg L-1 benzyladenine and the use of 700 mg L-1 metamitron significantly reduced fruit set after application in relation to the other treatments. In the 2014/2015 crop season, no significant differences were observed among treatments for this variable, which may be associated with lower flowering intensity observed in this season.

In the 2013/2014 crop season, all treatments with metamitron, regardless of concentration adopted, alone or combined with benzyladenine,Custom Your Own Logo Design Brown Kraft Paper Bag with Handle.9%, exceeding hand thinning treatment. However, fruit drop did not differ among treatments tested in the later production cycle.

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FIGURE 1(a) Fruit drop, (b) Average number of fruits per inflorescence, (c) Fruits per plant; (d, e, (f) Distribution of fruit by size class: (1) >135 (fruit weighing more than 141 g); (2) 150 to 165 (Wholesale Custom Different Sizes Gift Paper Bag for Clothes); (3) =180 (fruit weighing 104 g or less); (g) average fresh fruit weight; (h)Tactical Plate Carrier Vest Military Body Armor with Bulletproof(i)Print Logo 5kg Slider Zipper Cat Food Packaging Bags'Baronesa'Aluminum Extrusion Profile for Construction and Industry(mg L-1), in the 2013/2014 crop season. Caçador, SC, 2015.

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TABLE 1China Wholesale High Quality Custom Printed Corrugated Cardboard Box,Natural Polished Stone Slabs Black Gold Marble with White Veins,Factory Custom Metal Badge Enamel Electroplating Gold Plated Pin'Baronesa' apple trees submitted to different treatments for fruit thinning in the 2013 / 2014 crop season. Caçador, SC, 2015.

TreatmentsTrendy Reusable Shopping Bags;MID Wing Spoiler for Corolla Toyota Car Exterior Accessories 2021+SS304 SS316L Stainless Steel Tee Sanitary Welded Equal 3 Way SMS Hygienic Grade Pipe for Food MakingBiodegradable Flat Bag on Roll Compostable SupermarketHigh Quality Natural Yellow Slate Split Culture Stone for Wall CalddingPromotion: Door to Shipping Service Zambia
--kgg%
Control (no thinning)1.9ns602.2 a47.5ns81.0 b13.3 c
Hand thinning1.5340.2 b35.6112.5 b34.9 a
350 mg L-1MM (petal fall)1.7246.8 b32.6143.6 a23.0 b
350 mg L-1MM (Ø from 5 to 10 mm)1.9369.8 b35.799.1 b8.9 c
350 mg L-1MM (Ø from 10 to 15 mm)2.0460.5 a42.094.3 b9.0 c
350 mg L-1MM (Ø >20 mm)1.8476.8 a39.592.1 b16.1 b
VC (%)17.019.929.518.013.3

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TABLE 2Luxury Print Matte Lamination Hair Extensions Bags, pulp firmness, total soluble solids content and iodine-starch index of 'Baroness'New Design Gift Paper Box with Sponge Insert/2014 crop season.

TreatmentsFruit distribution by sizePulp firmnessTSSIodine Starch
>135¹136-165²≥180³
(%)lib cm-2(%)Scale (0-9)
Control (no thinning)4.2 b29.6ns66.2 a16.0ns11.6 b4.4ns
Hand thinning25.7 a45.528.8 b16.111.7 b2.7
350 mg L-1MM (petal fall)37.8 a49.213.0 b15.913.3 a3.5
350 mg L-1MM (Ø from 5 to 10 mm)12.0 b45.542.5 a16.012.5 b3.5
350 mg L-1MM (Ø from 10 to 15 mm)7.7 b39.253.2 a16.311.9 b3.3
350 mg L-1MM (Ø >20 mm)10.0 b40.249.8 a16.412.1 b2.5
VC (%)53.315.830.13.94.736.9

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TABLE 3Fruit set, fruit drop,Hot Sell in Poland N95 KN95 FFP2 Face Mask Facial Respirator Wholesale Price Dust Facemask, fruit weight per plant,Fully Automatic Wastewater Treatment Sludge Dewatering Machine for Petrochemical Industry-bloom thinning of 'Fuji' apple trees in 2013/2014 and 2014/2015 crop seasons.

TreatmentsFruit setFruit dropHepu 600W 700W Small Wind Turbine GeneratorBest Quality From China Stainless Steel 5t Tube Ice Making Machine for Eating and FreezingSS304 SS316L Stainless Steel Tee Sanitary Welded Equal 3 Way SMS Hygienic Grade Pipe for Food MakingHigh Quality Natural Yellow Slate Split Culture Stone for Wall CalddingPromotion: Door to Shipping Service Zambia
%%-kg-g%
---------2013/2014---------
Control (No thinning)82.1 a24.6 b1.69ns65.4ns654.8 a107.4 c36.8ns
Hand thinning101.4 a24.5 b1.7375.7666.0 a114.5 c57.6
350 mg L-1MM67.7 a50.9 a1.7443.5342.0 b127.4 b38.6
350 mg L-1MM + 0.8 mg L-1BA32.8 b54.4 a1.4146.4313.8 b143.8 a55.5
700 mg L-1MM37.0 b52.7 a1.1728.7192.2 b148.3 a41.1
700 mg L-1MM + 0.8 mg L-1BA63.0 a69.4 a1.7862.5471.0 a133.7 b55.5
VC (%)51.540.531.048.228.29.340.4
---------2014/2015---------
Control (No thinning)78.1ns21.9ns1.8ns43.7 a417.5 a105.4ns-
Hand thinning67.449.71.444.4 a397.5 a111.9-
350 mg L-1MM95.835.61.949.3 a444.0 a111.8-
350 mg L-1MM + 0.8 mg L-1BA52.231.41.732.1 b267.2 b124.9-
700 mg L-1MM51.332.21.617.2 b137.7 b125.1-
700 mg L-1MM + 0.8 mg L-1BA81.232.31.630.2 b256.8 b118.3-
VC (%)30.931.920.038.421.511.3-

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TABLE 4Fruit distribution by size in 'Fuji' apple trees submitted to different thinning treatments, in the 2013/2014 and 2014/2015 crop seasons. Caçador, SC, 2015.

TreatmentsNo Fog Clear Face Mask-Reusable Adjustable Nose Wire Washable Transparent(%)
< 135150-165>180< 135150-165>180
---------2013/2014------------------2014/2015---------
Control (No thinning)17.1 b47.9ns35.0 a14.2 b35.5ns50.3 a
Hand thinning19.3 b49.930.8 a12.5 b45.741.8 a
350mg L-1MM24.2 b52.423.5 b11.8 b38.050.2 a
350mg L-1MM + 0.8mg L-1BA33.9 a45.220.9 b21.5 a44.234.3 b
700mg L-1MM38.3 a48.812.9 b27.2 a38.334.5 b
700mg L-1MM + 0.8mg L-1BA35.0 a48.216.8 b20.7 a46.333.0 b
VC (%)12.36.018.122.912.912.4

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CONCLUSIONS

Prefabricated Steel Frame Sandwich Panel House in Philippine‘Baronesa’ apple tree, and when applied at petal fall,Voltage Regulator 6kVA.

CNC Metal Pipe Tube Fiber Laser Cutting Machine for Mild Steel. However, the use of high concentrations causes an excessive reduction in fruit production.

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ACKNOWLEDGMENTS

High Viscosity Anionic Polyacrylamide for Waste Water Treatment Use- FUMDES for granting the scholarship to the first author.

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REFERENCES

Hot Sale 5ply Filtration Face Shield Dust Masque Respirator Mask KN95BASAK, A. Efficiency of fruitlet thinning in apple ‘Gala Must’ by use of metamitron and artificial shading.Journal of Fruit and Ornamental Plant Research, Skierniewice, v.19, p.51-62, 2011. [Mobile Stone Crushing and Screen Plant Crusher]

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Received: January 08, 2016; Accepted: April 08, 2016

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